| Autor: |
Hendrickson OD; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071, Moscow, Russia. dzantiev@inbi.ras.ru., Zvereva EA; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071, Moscow, Russia. dzantiev@inbi.ras.ru., Solopova ON; Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye Shosse 24, 115478, Moscow, Russia., Varlamov NE; Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye Shosse 24, 115478, Moscow, Russia., Shemchukova OB; Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye Shosse 24, 115478, Moscow, Russia., Zherdev AV; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071, Moscow, Russia. dzantiev@inbi.ras.ru., Sveshnikov PG; Russian Research Center for Molecular Diagnostics and Therapy, Sympheropolsky Blvrd., 8, 117638, Moscow, Russia., Dzantiev BB; Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071, Moscow, Russia. dzantiev@inbi.ras.ru. |
| Abstrakt: |
A lateral flow immunoassay (LFIA) of phycotoxin domoic acid (DA) contaminating seawater and marine organisms was developed in this investigation. Nine clones of monoclonal antibodies against DA were produced and characterized. The test system was implemented in the indirect competitive format, where gold nanoparticles as a marker were conjugated with secondary antibodies. The developed test system allows for the detection of DA with a cutoff of 60 ng mL -1 and an instrumental detection limit of 1.4 ng mL -1 within 15 min. The LFIA was applied to detect DA in seawater, mussels, shrimps, and octopuses. A simple method of seafood sample preparation was proposed. The entire analytical cycle, from obtaining a sample to the estimation of final results, takes only 30 min. The assay recoveries ranged from 88.5% to 124%. The developed analytical method is a promising solution for rapid on-site monitoring of marine toxicants in water and food throughout the farm-to-fork chain. |
