Periodate oxidation of plant polysaccharides provides polysaccharide-specific oligosaccharides.

Autor: Pandeirada CO; Wageningen University & Research, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands., Achterweust M; Wageningen University & Research, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands., Janssen HG; Unilever Foods Innovation Centre - Hive, Bronland 14, 6708 WH Wageningen, the Netherlands; Wageningen University & Research, Laboratory of Organic Chemistry, P.O. Box 8026, 6700 EG Wageningen, the Netherlands., Westphal Y; Unilever Foods Innovation Centre - Hive, Bronland 14, 6708 WH Wageningen, the Netherlands., Schols HA; Wageningen University & Research, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands. Electronic address: henk.schols@wur.nl.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2022 Sep 01; Vol. 291, pp. 119540. Date of Electronic Publication: 2022 Apr 27.
DOI: 10.1016/j.carbpol.2022.119540
Abstrakt: Although polysaccharides are frequently used in foods, detailed characterization and/or identification of their structures using a single method remains a challenge. We investigated the suitability of periodate oxidation as an approach to depolymerize polysaccharides, allowing characterization and/or identification of the original polysaccharides based on ESI-MS analyses of the released oligosaccharides. Various periodate oxidation conditions were tested on (arabino)xylan, galactomannan, xyloglucan and homogalacturonan. Each polysaccharide required a different oxidation condition to release a substantial level of oligosaccharides. These oligosaccharides had highly complex structures due to the presence of e.g., dialdehyde sugars, hemialdals, and remnants of (oxidized) sugars, as verified by ESI-MS/MS. Despite these oligosaccharides were highly complex and lost some polysaccharide structural features, each periodate-oxidized sample comprised polysaccharide structure-dependent MS oxidized oligosaccharide profiles. Our findings are a good starting point to find a more generic chemical polysaccharide depolymerization approach based on periodate oxidation to identify polysaccharides by oligosaccharides fingerprinting using MS.
(Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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