Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues.

Autor: Wang M; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Li W; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Hao J; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Gonzales A 3rd; University of the Philippines Diliman, Quezon City, Metro Manila, Philippines., Zhao Z; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Flores RS; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Kuang X; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Mu X; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Ching T; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.; Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore, Singapore.; Digital Manufacturing and Design Centre, Singapore University of Technology and Design, Singapore, Singapore.; Department of Biomedical Engineering, National University of Singapore, Singapore, Singapore., Tang G; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Luo Z; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Garciamendez-Mijares CE; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Sahoo JK; Department of Biomedical Engineering, Tufts University, Medford, MA, USA., Wells MF; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Niu G; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA., Agrawal P; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA., Quiñones-Hinojosa A; Departments of Neurosurgery, Oncology, Neuroscience, Mayo Clinic, Jacksonville, FL, USA., Eggan K; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Zhang YS; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. yszhang@research.bwh.harvard.edu.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. yszhang@research.bwh.harvard.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jun 09; Vol. 13 (1), pp. 3317. Date of Electronic Publication: 2022 Jun 09.
DOI: 10.1038/s41467-022-31002-2
Abstrakt: Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication with this method remains a challenge to balance the physical performances of the bioinks for high-fidelity bioprinting and suitable microenvironments for the encapsulated cells to thrive. Here, we propose a molecular cleavage approach, where hyaluronic acid methacrylate (HAMA) is mixed with gelatin methacryloyl to achieve high-performance bioprinting, followed by selectively enzymatic digestion of HAMA, resulting in tissue-matching mechanical properties without losing the structural complexity and fidelity. Our method allows cellular morphological and functional improvements across multiple bioprinted tissue types featuring a wide range of mechanical stiffness, from the muscles to the brain, the softest organ of the human body. This platform endows us to biofabricate mechanically precisely tunable constructs to meet the biological function requirements of target tissues, potentially paving the way for broad applications in tissue and tissue model engineering.
(© 2022. The Author(s).)
Databáze: MEDLINE
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