Diversity assessment of photosynthesizers: comparative analysis of pre-cultivated and natural microbiome of sediments from Cerrado biome in Maranhão, Brazil.

Autor: Butarelli ACA; Department of Biology, Center for Biological and Health Sciences, Federal University of Maranhão, Cidade Universitária Dom Delgado, Av. dos Portugueses, 1966,Vila Bacanga, São Luís, MA, 65080-805, Brazil.; Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanográfico, 191, Cidade Universitária, São Paulo, SP, 05508-120, Brazil., Ferreira LSS; Department of Biology, Center for Biological and Health Sciences, Federal University of Maranhão, Cidade Universitária Dom Delgado, Av. dos Portugueses, 1966,Vila Bacanga, São Luís, MA, 65080-805, Brazil.; Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanográfico, 191, Cidade Universitária, São Paulo, SP, 05508-120, Brazil., Riyuzo R; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, Cidade Universitária, São Paulo, SP, 05508-000, Brazil., Dall'Agnol HMB; Department of Pathology, Center for Biological and Health Sciences, Federal University of Maranhão, Cidade Universitária Dom Delgado, Av. dos Portugueses, 1966, Vila Bacanga, São Luís, MA, 65080-805, Brazil., Piroupo CM; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, Cidade Universitária, São Paulo, SP, 05508-000, Brazil., da Silva AM; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, Cidade Universitária, São Paulo, SP, 05508-000, Brazil., Setubal JC; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, Cidade Universitária, São Paulo, SP, 05508-000, Brazil., Dall'Agnol LT; Department of Biology, Center for Biological and Health Sciences, Federal University of Maranhão, Cidade Universitária Dom Delgado, Av. dos Portugueses, 1966,Vila Bacanga, São Luís, MA, 65080-805, Brazil. leonardo.td@ufma.br.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 Nov; Vol. 29 (51), pp. 77359-77374. Date of Electronic Publication: 2022 Jun 08.
DOI: 10.1007/s11356-022-21229-3
Abstrakt: Photosynthetic microorganisms are important components of most ecosystems and have important roles regarding biogeochemical cycles and the basis of the trophic chain. However, they sometimes are present in low abundance compared to other heterotrophic organisms. The Chapada das Mesas National Park (PNCM) is a Conservation Unit in Brazilian Cerrado biome, which is considered a hotspot for biodiversity conservation and possesses important rivers, waterfalls, and springs with economical and touristic importance. The aim of this study was to perform a comparative analysis of enriched and total microbiome of sediments to understand the impact of pre-cultivation in discovery of underrepresented groups like photosynthesizers. All sediment samples were cultivated in BG-11 medium under illumination to enrich for photosynthetic microorganisms and both the raw samples and the enriched ones were submitted to DNA extraction and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene on the Ion Torrent platform. The reads were analyzed using QIIME2 software and the Phyloseq package. The enrichment allowed detection and identification of many genera of cyanobacteria in the Chapada das Mesas National Park (PNCM), which would probably not be possible without the combination of approaches. A total of 58 groups of photosynthetic microorganisms were classified in the samples from the enrichments and their relative abundance based on amplified 16S rRNA sequences were estimated, highlighting the genus Synechocystis which represented 10.10% of the abundance of the phylum Cyanobacteria and the genus Dunaliella, which represented 45.66% of the abundance of algae as the most abundant groups at the PNCM. In the enrichments, microorganisms from the phyla Proteobacteria (45.2%), Bacteroidetes (18%), and Planctomycetes (3.3%) were also identified, since there are ecological associations between the photosynthetic community and other groups of heterotrophic microorganisms. As for the functional analysis, metabolic functions associated with methanotrophy and methylotrophy, hydrocarbon degradation, phototrophy, and nitrogen fixation were predicted. The results highlight a great diversity of photosynthetic microorganisms in Cerrado and the importance of using a combination of approaches when analyzing target groups which are usually underrepresented such as cyanobacteria and microalgae.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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