In situ-Synthesized cadmium sulfide quantum dots in pore-forming protein and polysaccharide matrices for optical biosensing applications.

Autor: Sergeev AA; Institute of Automation and Control Processes, Far Eastern Branch of the Russian Academy of Sciences, 5, Radio street, Vladivostok 690041, Russia., Naberezhnykh GA; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159, prospect 100 Let Vladivostoku, Vladivostok 690022, Russia., Khomenko VA; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159, prospect 100 Let Vladivostoku, Vladivostok 690022, Russia., Amosov AV; Institute of Automation and Control Processes, Far Eastern Branch of the Russian Academy of Sciences, 5, Radio street, Vladivostok 690041, Russia., Nepomnyaschiy AV; Institute of Automation and Control Processes, Far Eastern Branch of the Russian Academy of Sciences, 5, Radio street, Vladivostok 690041, Russia., Solov'eva TF; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159, prospect 100 Let Vladivostoku, Vladivostok 690022, Russia., Chistyulin DK; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159, prospect 100 Let Vladivostoku, Vladivostok 690022, Russia., Tutov MV; Far Eastern Federal University, 10, Ajax Bay, Russky Island, Vladivostok 690922, Russia., Kulchin YN; Institute of Automation and Control Processes, Far Eastern Branch of the Russian Academy of Sciences, 5, Radio street, Vladivostok 690041, Russia., Novikova OD; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159, prospect 100 Let Vladivostoku, Vladivostok 690022, Russia.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2022 Sep; Vol. 217, pp. 112607. Date of Electronic Publication: 2022 May 31.
DOI: 10.1016/j.colsurfb.2022.112607
Abstrakt: The main limitation for practical implementation of quantum dots-based sensors and biosensors is the possible contamination of sensing media with quantum dots (QDs) moved out from the sensor structure, being critical for living systems measurements. Numerous efforts have addressed the challenge of pre-synthesized QDs incorporation into porous matrix provide, on the one hand, proper fixation of quantum dots in its volume and preserving a free analyte transfer from the sensing media to them - on the other hand. Here, we propose an alternative insight into this problem. Instead of using preliminary synthesized particles for doping a matrix, we have in situ synthesized cadmium sulfide QDs in porous biopolymeric matrices, both in an aqueous solution and on a mica substrate. The proposed technique allows obtaining QDs in a matrix acting simultaneously as a ligand passivating surface defects and preventing QDs aggregation. The conjugates were used as a photoluminescence sensor for the metal ions and glutathione detection in an aqueous media. Different kinds of sensor responses have been found depending on the analyte nature. Zinc ions' presence initiates the intraband QDs emission increases due to the reduction of non-radiative processes. The presence of copper ions, in contrast, leads to a gradual photoluminescence decrease due to the formation of the non-luminescent copper-based alloy in the QDs structure. Finally, the presence of glutathione initiates a ligand exchange process followed by some QDs surface treatment enhancing defect-related photoluminescence. As a result, three different kinds of sensor responses for three analytes allow claiming development of a new selective QD-based sensor suitable for biomedical applications.
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Databáze: MEDLINE
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