| Autor: |
Ekonomou SI; Faculty of Health and Applied Sciences (HAS), University of the West of, Coldharbour Ln, Bristol BS16 1QY, England., Akshay Thanekar P; Faculty of Health and Applied Sciences (HAS), University of the West of, Coldharbour Ln, Bristol BS16 1QY, England., Lamprou DA; School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK., Weaver E; School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK., Doran O; Faculty of Health and Applied Sciences (HAS), University of the West of, Coldharbour Ln, Bristol BS16 1QY, England., Stratakos AC; Faculty of Health and Applied Sciences (HAS), University of the West of, Coldharbour Ln, Bristol BS16 1QY, England. |
| Abstrakt: |
Salmonella is a global health threat, with pig production being one of the main sources of human salmonellosis. The current study investigated the antivirulence properties of geraniol for inhibiting the in vitro colonization of Salmonella . The minimum inhibitory (MIC) and bactericidal concentrations (MBC) of geraniol against Salmonella typhimurium followed by the sub-MIC of geraniol were determined. Results provided clear evidence that geraniol at 1/8 MIC can be used as an effective, non-toxic antivirulence compound to inhibit virulence factors (motility, adhesion, and invasiveness) affecting the colonization of S. typhimurium on IPEC-J2 cells. Additionally, the findings signified that microfluidics is an emerging technology suitable for the preparation of stable liposomes with a small size (<200 nm) and high encapsulation efficiency (EE) of up to 92.53%, which can act as effective carriers of geraniol into the pig gastrointestinal tract (GIT), targeting Salmonella , preventing colonization, and thus increasing the safety of the food supply chain. |
