A new technique to evaluate Acidithiobacillus thiooxidans growth during a bioleaching process based on DNA quantification.

Autor: Rivas-Castillo AM; Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Unidad Querétaro, Cerro Blanco 141, Col. Colinas del Cimatario, 76090 Santiago de Querétaro, Qro, Mexico; Universidad Tecnológica de la Zona Metropolitana del Valle de México, Miguel Hidalgo y Costilla 5, Col. Los Héroes de Tizayuca, 43816 Tizayuca, Hgo, Mexico. Electronic address: a.rivas@utvam.edu.mx., Gómez-Ramírez M; Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Unidad Querétaro, Cerro Blanco 141, Col. Colinas del Cimatario, 76090 Santiago de Querétaro, Qro, Mexico. Electronic address: mgomezr@ipn.mx., Lucas-Gómez IM; Universidad Tecnológica de la Zona Metropolitana del Valle de México, Miguel Hidalgo y Costilla 5, Col. Los Héroes de Tizayuca, 43816 Tizayuca, Hgo, Mexico. Electronic address: i.lucas@utvam.edu.mx., Carrillo-Vega Y; Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Unidad Querétaro, Cerro Blanco 141, Col. Colinas del Cimatario, 76090 Santiago de Querétaro, Qro, Mexico., Rojas-Avelizapa NG; Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del IPN, Unidad Querétaro, Cerro Blanco 141, Col. Colinas del Cimatario, 76090 Santiago de Querétaro, Qro, Mexico. Electronic address: nrojasa@ipn.mx.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2022 Jul; Vol. 198, pp. 106494. Date of Electronic Publication: 2022 May 26.
DOI: 10.1016/j.mimet.2022.106494
Abstrakt: The potential of Acidithiobacillus (Thiobacillus) genus members, namely Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, for bioleaching purposes is known. Specifically, previous studies have shown the potential of A. thiooxidans strain DSM 26636 used in bioleaching processes to remove metals in high-metal-content matrices. All Acidithiobacillus growth-monitoring techniques available to date, including sulfate production, commonly used, present disadvantages. Thus, the current work shows a technique based on DNA quantification to evaluate the growth of A. thiooxidans DSM 26636, which is useful even in the presence of a high-metal-content residue. This proposed methodology may represent a functional complementary tool to evaluate Acidithiobacillus growth to develop biometallurgical applications.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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