Routes to roots: direct evidence of water transport by arbuscular mycorrhizal fungi to host plants.

Autor: Kakouridis A; University of California Berkeley, Berkeley, CA, 94720, USA.; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Hagen JA; University of California Berkeley, Berkeley, CA, 94720, USA., Kan MP; University of California Berkeley, Berkeley, CA, 94720, USA.; Lawrence Livermore National Laboratory, Livermore, CA, 94550, USA., Mambelli S; University of California Berkeley, Berkeley, CA, 94720, USA.; Center for Stable Isotope Biogeochemistry, Berkeley, CA, 94720, USA., Feldman LJ; University of California Berkeley, Berkeley, CA, 94720, USA., Herman DJ; University of California Berkeley, Berkeley, CA, 94720, USA.; Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Weber PK; Lawrence Livermore National Laboratory, Livermore, CA, 94550, USA., Pett-Ridge J; Lawrence Livermore National Laboratory, Livermore, CA, 94550, USA.; University of California Merced, Merced, CA, 95344, USA., Firestone MK; University of California Berkeley, Berkeley, CA, 94720, USA.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2022 Oct; Vol. 236 (1), pp. 210-221. Date of Electronic Publication: 2022 Jul 01.
DOI: 10.1111/nph.18281
Abstrakt: Arbuscular mycorrhizal fungi (AMF) can help mitigate plant responses to water stress, but it is unclear whether AMF do so by indirect mechanisms, direct water transport to roots, or a combination of the two. Here, we investigated if and how the AMF Rhizophagus intraradices transported water to the host plant Avena barbata, wild oat. We used two-compartment microcosms, isotopically labeled water, and a fluorescent dye to directly track and quantify water transport by AMF across an air gap to host plants. Plants grown with AMF that had access to a physically separated compartment containing 18 O-labeled water transpired almost twice as much as plants with AMF excluded from that compartment. Using an isotopic mixing model, we estimated that water transported by AMF across the air gap accounted for 34.6% of the water transpired by host plants. In addition, a fluorescent dye indicated that hyphae were able to transport some water via an extracytoplasmic pathway. Our study provides direct evidence that AMF can act as extensions of the root system along the soil-plant-air continuum of water movement, with plant transpiration driving water flow along hyphae outside of the hyphal cell membrane.
(© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)
Databáze: MEDLINE
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