Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust.

Autor:	Colton H; South Yorkshire Regional Department of Infection and Tropical Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Hodgson D; Centre for Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK., Hornsby H; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Brown R; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Mckenzie J; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Bradley KL; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., James C; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Lindsey BB; South Yorkshire Regional Department of Infection and Tropical Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Birch S; Academic Directorate of Communicable Diseases and Specialised Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK., Marsh L; Academic Directorate of Communicable Diseases and Specialised Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK., Wood S; Department of Scientific Computing and Informatics, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK., Bayley M; Department of Scientific Computing and Informatics, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK., Dickson G; Department of Scientific Computing and Informatics, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK., James DC; Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, S10 2TN, UK., Nicklin MJ; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Sayers JR; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK.; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield, S10 2TN, UK.; Sheffield Institute for Nucleic Acids, University of Sheffield, Sheffield, S10 2TN, UK., Zafred D; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK., Rowland-Jones SL; South Yorkshire Regional Department of Infection and Tropical Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK.; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield, S10 2TN, UK., Kudesia G; Department of Virology, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S5 7AU, UK., Kucharski A; Centre for Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK., Darton TC; South Yorkshire Regional Department of Infection and Tropical Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK.; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield, S10 2TN, UK., de Silva TI; South Yorkshire Regional Department of Infection and Tropical Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK.; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield, S10 2TN, UK., Collini PJ; South Yorkshire Regional Department of Infection and Tropical Medicine, Sheffield Teaching Hospitals Nhs Foundation Trust, Sheffield, S10 2JF, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, S10 2TN, UK.; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield, S10 2TN, UK.
Jazyk:	angličtina
Zdroj:	Wellcome open research [Wellcome Open Res] 2022 Jun 10; Vol. 6, pp. 220. Date of Electronic Publication: 2022 Jun 10 (Print Publication: 2021).
DOI:	10.12688/wellcomeopenres.17143.3
Abstrakt:	Background: We aimed to measure SARS-CoV-2 seroprevalence in a cohort of healthcare workers (HCWs) during the first UK wave of the COVID-19 pandemic, explore risk factors associated with infection, and investigate the impact of antibody titres on assay sensitivity. Methods:  HCWs at Sheffield Teaching Hospitals NHS Foundation Trust were prospectively enrolled and sampled at two time points. We developed an in-house ELISA for testing participant serum for SARS-CoV-2 IgG and IgA reactivity against Spike and Nucleoprotein. Data were analysed using three statistical models: a seroprevalence model, an antibody kinetics model, and a heterogeneous sensitivity model. Results:  Our in-house assay had a sensitivity of 99·47% and specificity of 99·56%. We found that 24·4% (n=311/1275) of HCWs were seropositive as of 12th June 2020. Of these, 39·2% (n=122/311) were asymptomatic. The highest adjusted seroprevalence was measured in HCWs on the Acute Medical Unit (41·1%, 95% CrI 30·0-52·9) and in Physiotherapists and Occupational Therapists (39·2%, 95% CrI 24·4-56·5). Older age groups showed overall higher median antibody titres. Further modelling suggests that, for a serological assay with an overall sensitivity of 80%, antibody titres may be markedly affected by differences in age, with sensitivity estimates of 89% in those over 60 years but 61% in those ≤30 years. Conclusions:   HCWs in acute medical units and those working closely with COVID-19 patients were at highest risk of infection, though whether these are infections acquired from patients or other staff is unknown. Current serological assays may underestimate seroprevalence in younger age groups if validated using sera from older and/or more severe COVID-19 cases. Competing Interests: No competing interests were disclosed. (Copyright: © 2022 Colton H et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu