| Autor: |
Borghi-Pangoni FB; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Brazil., Bassi da Silva J; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Brazil., Dos Santos RS; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Brazil., Trevisan AP; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Brazil., Hott FCC; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Brazil., Gonçalves MC; Laboratory of Basic and Applied Bacteriology NIP3, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., Kobayashi RKT; Laboratory of Basic and Applied Bacteriology NIP3, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil., de Souza MVF; Laboratory of Clinical Cytology, Building B09, Department of Clinical Analysis, Center of Health Sciences, State University of Maringa, Maringa, Brazil., Consolaro MEL; Laboratory of Clinical Cytology, Building B09, Department of Clinical Analysis, Center of Health Sciences, State University of Maringa, Maringa, Brazil., Castro-Hoshino LV; Postgraduate Program in Physics, Department of Physics, State University of Maringa, Maringa, Brazil., Baesso ML; Postgraduate Program in Physics, Department of Physics, State University of Maringa, Maringa, Brazil., Bruschi ML; Laboratory of Research and Development of Drug Delivery Systems, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy, State University of Maringa, Maringa, Brazil. |
| Abstrakt: |
Thermosensitive bioadhesive formulations can display increased retention time, skin permeation, and improve the topical therapy of many drugs. Acne is an inflammatory process triggered by several factors like the proliferation of the bacteria Propionibacterium acnes . Aiming for a new alternative treatment with a natural source, propolis displays great potential due to its antibiotic, anti-inflammatory, and healing properties. This study describes the development of bioadhesive thermoresponsive platform with cellulose derivatives and poloxamer 407 for propolis skin delivery. Propolis ethanolic extract (PES) was added to the formulations with sodium carboxymethylcellulose (CMC) or hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (Polox). The formulations were characterized as rheology, bioadhesion, and mechanical analysis. The selected formulations were investigated as in vitro propolis release, cytotoxicity, ex vivo skin permeation by Fourier Transform Infrared Photoacoustic Spectroscopy, and the activity against P. acnes . Formulations showed suitable sol-gel transition temperature, shear-thinning behavior, and texture profile. CMC presence decreased the cohesiveness and adhesiveness of formulations. Polox/HPMC/PES system displayed less cytotoxicity, modified propolis release governed by anomalous transport, skin permeation, and activity against P. acnes . These results indicate important advantages in the topical treatment of acne and suggest a potential formulation for clinical evaluation. |
