| Autor: |
Sommerfeld S; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Mundim AV; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Silva RR; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Queiroz JS; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Rios MP; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Notário FO; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Medeiros Ronchi AA; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil., Beletti ME; Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405-319, Brazil., Franco RR; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia 38405-319, Brazil., Espindola FS; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia 38405-319, Brazil., Goulart LR; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia 38405-319, Brazil., Fonseca BB; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38402-018, Brazil.; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia 38405-319, Brazil. |
| Abstrakt: |
Several studies have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardize the evaluated parameters are needed to better understand and identify the viability of CEs as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are specific to the model. To achieve this goal, in ovo assays were performed by injecting a virus ( Gammacoronavirus ) and two drugs (filgrastim and dexamethasone) that cause known changes in adult animals. Although congestion and inflammatory infiltrate were visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not increase granulocytes as we expected. On the other hand, CE weight and red blood cell count were lower with dexamethasone (DX), whereas white blood cell count and biomarkers varied depended on the stage of CE development. Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are reproducible. |
