| Autor: |
Pardesi B; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Roberton AM; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Wollmuth EM; Department of Microbiology, Cornell University, Ithaca, NY 14853, USA., Angert ER; Department of Microbiology, Cornell University, Ithaca, NY 14853, USA., Rosendale DI; Anagenix Ltd, Parnell, Auckland 1052, New Zealand., White WL; School of Science, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand., Clements KD; School of Biological Sciences, University of Auckland, Auckland, New Zealand. |
| Abstrakt: |
A Gram-stain-positive, non-spore-forming, rod-shaped, obligately anaerobic bacterium, designated strain BP52G T , was isolated from the hindgut of a Silver Drummer ( Kyphosus sydneyanus ) fish collected from the Hauraki Gulf, New Zealand. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belonged to the family Erysipelotrichaceae in the phylum Firmicutes and was most closely related to Clostridium saccharogumia with 93.3 % sequence identity. Isolate BP52G T grew on agar medium containing mannitol as the sole carbon source. White, opaque and shiny colonies of the isolate measuring approximately 1 mm diameter grew within a week at 20-28 °C (optimum, 24 °C) and pH 6.9-8.5 (optimum, pH 7.8). BP52G T tolerated the addition of up to 1 % NaCl to the medium. Formate and acetate were the major fermentation products. The major cellular fatty acids were C 16 : 0 , C 16:1n-7t and C 18:1n-7t . The genome sequence of the isolate was determined. Its G+C content was 30.7 mol%, and the 72.65 % average nucleotide identity of the BP52G T genome to its closest neighbour with a completely sequenced genome ( Erysipelatoclostridium ramosum JCM 1298 T ) indicated low genomic relatedness. Based on the phenotypic and taxonomic characteristics observed in this study, a novel genus and species Tannockella kyphosi gen. nov., sp. nov. is proposed for isolate BP52G T (=NZRM 4757 T =JCM 34692 T ). |
