| Autor: |
Lei RF; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, PR China., Ma Q; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, PR China., Li YQ; CAS Key Laboratory of Tropical Marine Bio Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 511458, PR China., Abudourousuli D; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, PR China., Wang S; Heilongjiang Academy of Black Soil Conservation & Utilization, Heilongjiang Academy of Agricultural Sciences, Key Lab of Soil Environment and Plant Nutrition of Heilongjiang Province, Heilongjiang Fertilizer Engineering Research Center, Harbin, 150086, PR China., Rouzi Z; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, PR China., Aosiman M; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, PR China., An DD; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, 830054, PR China., Li WJ; State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, PR China. |
| Abstrakt: |
A bacterial strain, designated YZJH907-2 T , was isolated from the stem of Suaeda aralocaspica , collected from the southern edge of the Gurbantunggut desert, Xinjiang, PR China. Cells of strain YZJH907-2 T were Gram-stain-positive, aerobic and rod-shaped. They formed white or colourless circular colonies with smooth convex surfaces. Strain YZJH907-2 T grew at 4-50 °C (optimum, 28-30 °C), pH 7.0-10.0 (optimum, pH 8.0-9.0) and with 0-10 % (w/v) NaCl (optimum, 3-7 %). The genomic DNA G+C content of strain YZJH907-2 T was 38.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the strain was most closely related to Bacillus alcalophilus DSM 485 T (97.37 %), Bacillus kiskunsagensis B16-24 T (96.87 %) and Bacillus bogoriensis LBB3 T (96.71 %). Average nucleotide identity values between YZJH907-2 T and B. alcalophilus DSM 485 T and B. bogoriensis LBB3 T were 69.2 and 69.0 %, respectively. Digital DNA-DNA hybridization values of YZJH907-2 T with B. alcalophilus DSM 485 T and B. bogoriensis LBB3 T were 19.6 and 20.4 %, respectively. The cell wall of strain YZJH907-2 T contained meso -diaminopimelic acid, and the major and secondary isoprenoid quinones were MK-7 and MK-5, respectively. Results of fatty acids showed that anteiso-C 15 : 0 , iso-C 15 : 0 and C 16 : 0 were the predominant cellular fatty acids. Two-dimensional thin-layer chromatography analysis indicated that the polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. Based on the genomic, phylogenetic and phenotypic analyses, strain YZJH907-2 T represented a novel species of the genus Bacillus , and thus the name Bacillus suaedae sp. nov. is proposed. The type strain is YZJH907-2 T (=CGMCC 1.18763 T =KCTC 43335 T ). |
