Evaluation of immune responses and oxidative stress in donkeys: Immunological studies provoked by Parascaris equorum infection.

Autor:	Attia MM; Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt., Soliman SM; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt., Salaeh NMK; Zoology Department, Faculty of Science, Aswan University, Aswan, Egypt., Salem HM; Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt., Alkafafy M; Department of Biotechnology, College of Science, Taif University. P.O.Box 11099, Taif 21944 Saudi Arabia., Saad AM; Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt., El-Saadony MT; Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt., El-Gameel SM; Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt.
Jazyk:	angličtina
Zdroj:	Saudi journal of biological sciences [Saudi J Biol Sci] 2022 Apr; Vol. 29 (4), pp. 2173-2179. Date of Electronic Publication: 2021 Nov 24.
DOI:	10.1016/j.sjbs.2021.11.044
Abstrakt:	This study aimed to assess the effects of Parascaris equorum ( P. equorum) in infected donkeys through evaluation the oxidative stress and different gene parameters in infected tissues. Fifty donkeys were examined in Giza Zoo abattoir from the period of January to March 2021. Blood and sera samples were collected from each examined donkey. P. equorum were subjected for identification through scanning electron microscope study and the infected tissues were subjected into gene expression analysis using two genes; interleukin 1β (IL1- β); and pro-inflammatory cytokines (TNF-α) with assessment of the antioxidant and free radicals released from the animals during the infection. Eighteen donkeys were positive for P. equorum adult or larvae by postmortem examination of the intestine and abdomen with prevalence rate of 36 %. The examined infected donkeys with P. equorum showed significantly higher of Total antioxidant capacity (TAC) levels and the serum malondialdehyde (MDA) 2.45 ± 0.53 than that in non-infected control donkeys. The levels of AST enzyme were 278.54 ± 0.45 while ALT enzyme was 14.97 ± 0.87 which was significantly higher than that of control negative donkeys. The infected donkeys with P. equorum showed significantly upregulation of the TNF-α and IL-1β which classify according to number of collected worms. The P. equorum infected donkeys exerted at least 100 eggs of parasite in feces. The fecal egg count was marked decreased after treatment with moxidectin. Moxidectin is considered a novel active ingredient that has a marvelous result with high persistency and protection for long time, in addition to, broad spectrum activity and low or no resistance. We recommend the periodical deworming with different molecules as more economic and lifesaving over a single treatment every 12 months parallel with parasitic testing. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s).)
Databáze:	MEDLINE
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