Remarkably selective biocompatible turn-on fluorescent probe for detection of Fe 3+ in human blood samples and cells.
| Autor: | V H V; Center for Nano and Material Science, Jain University Jakkasandra Post Bangalore Rural-562112 India br.geetha@jainuniversity.ac.in., Saxena M; Center for Nano and Material Science, Jain University Jakkasandra Post Bangalore Rural-562112 India br.geetha@jainuniversity.ac.in., R GB; Center for Nano and Material Science, Jain University Jakkasandra Post Bangalore Rural-562112 India br.geetha@jainuniversity.ac.in., Latiyan S; Metallurgical and Materials Engineering, National Institute of Technology Tiruchirapalli-620015 India.; Centre for Biosystems Science and Engineering, Indian Institute of Science Bangalore-560012 India., Jain S; Centre for Biosystems Science and Engineering, Indian Institute of Science Bangalore-560012 India. |
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| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2019 Sep 02; Vol. 9 (47), pp. 27439-27448. Date of Electronic Publication: 2019 Sep 02 (Print Publication: 2019). |
| DOI: | 10.1039/c9ra05256a |
| Abstrakt: | The robust nature of a biocompatible fluorescent probe is demonstrated, by its detection of Fe 3+ even after repeated rounds of quenching (reversibility) by acetate in real human blood samples and cells in vitro . Significantly trace levels of Fe 3+ ions up to 8.2 nM could be detected, remaining unaffected by the existence of various other metal ions. The obtained results are validated by AAS and ICP-OES methods. A portable test strip is also fabricated for quick on field detection of Fe 3+ . As iron is a ubiquitous metal in cells and plays a prominent role in biological processes, the use of this probe to image Fe 3+ in cells is a substantial development towards biosensing. Cytotoxicity studies also proved the nontoxic nature of this probe. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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