A Combined Biomarker of Bright CD38 and MYC ≥55% Is Highly Predictive of Double-/Triple-Hit High-Grade B-Cell Lymphoma.

Autor:	Alsuwaidan A; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Koduru P; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Fuda F; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Manuel Jaso J; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Chen M; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Rosado F; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Luu HS; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Sweed N; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Garcia R; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Doucet M; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Desai NB; Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Kumar KA; Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA., Awan FT; Internal Medicine/Division of Hematologic Malignancies and Stem Cell Transplantation, University of Texas Southwestern Medical Center, Dallas, TX, USA., Ramakrishnan Geethakumari P; Internal Medicine/Division of Hematologic Malignancies and Stem Cell Transplantation, University of Texas Southwestern Medical Center, Dallas, TX, USA., Chen W; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Jazyk:	angličtina
Zdroj:	American journal of clinical pathology [Am J Clin Pathol] 2022 Sep 02; Vol. 158 (3), pp. 338-344.
DOI:	10.1093/ajcp/aqac047
Abstrakt:	Objectives: Diagnosis of high-grade B-cell lymphoma with MYC and BCL2 or BCL6 rearrangements (double-/triple-hit lymphoma [DTHL]) appears to mandate fluorescence in situ hybridization (FISH) testing for all large B-cell lymphoma (LBCL). Given the low incidence of DTHL, we aimed to identify flow cytometry (FC) and immunohistochemistry (IHC) features of DTHL that could be used to develop an optimal screening strategy. This combined FC-IHC approach has not yet been studied. Methods: We compared features of 40 cases of DTHL and 39 cases of diffuse LBCL (DLBCL) without MYC rearrangement. Results: Bright CD38 expression (CD38bright) by FC, high MYC expression (≥55%), and double-expressor phenotype by IHC were significantly associated with DTHL. The biomarker combining FC and IHC, CD38bright and/or MYC ≥55%, was superior to FC and IHC markers alone in predicting DTHL. Restricting FISH testing to approximately 25% of LBCL based on CD38brightand/or MYC ≥55% would detect approximately 95% of DTHL-BCL2 and approximately 75% of DHL-BCL6. Conclusions: Our study demonstrated that the novel biomarker of CD38bright and/or MYC ≥55% is highly predictive of DTHL. Awareness of the advantages and limitations of this screening strategy would facilitate development of a rational diagnostic workflow to provide high-quality patient care. (© The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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