[Intergeneric conjugational crossing of Escherichia coli with Salmonella typhimurium. II. Transfer of a polA1 mutation from Escherichia coli to Salmonella typhimurium and its phenotypic expression in the salmonella genome].

Autor: Stepanova NF, Andreeva IV, Kiriushkina AA, Kondrat'ev IuS, Likhoded LIa
Jazyk: ruština
Zdroj: Genetika [Genetika] 1977; Vol. 13 (12), pp. 2189-94.
Abstrakt: The Escherichia coli structural gene for DNA polymerase I was inserted into Salmonella typhimurium chromosome by conjugal transfer. The genetic analysis of P1-mediated transduction of obtained hybrid showed that polA gene is located in it between metE and rha loci and is cotransduced with metE (about 50%) and rha (12%). The phenotypic properties of polA1 hybrid E. coliXS. typhimurium concerning UV-MMS-NG and gamma-ray sensitivity are similar to the polA1 mutants of E. coli.
Databáze: MEDLINE