Longitudinal multi-omics analyses link gut microbiome dysbiosis with recurrent urinary tract infections in women.

Autor: Worby CJ; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., Schreiber HL 4th; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.; Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, USA.; Division of Biology & Biological Engineering, California Institute of Technology, Pasadena, CA, USA., Straub TJ; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., van Dijk LR; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA.; Delft Bioinformatics Lab, Delft University of Technology, Delft, the Netherlands., Bronson RA; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., Olson BS; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.; Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, USA., Pinkner JS; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA., Obernuefemann CLP; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA., Muñoz VL; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA., Paharik AE; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA., Azimzadeh PN; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA., Walker BJ; Applied Invention, Cambridge, MA, USA., Desjardins CA; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., Chou WC; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., Bergeron K; Department of Surgery, Division of Urologic Surgery, Washington University School of Medicine, St. Louis, MO, USA., Chapman SB; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., Klim A; Department of Surgery, Division of Urologic Surgery, Washington University School of Medicine, St. Louis, MO, USA., Manson AL; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA., Hannan TJ; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA., Hooton TM; Department of Medicine, University of Miami, Miami, FL, USA., Kau AL; Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, USA.; Department of Medicine, Division of Allergy and Immunology, Washington University School of Medicine, St. Louis, MO, USA., Lai HH; Department of Surgery, Division of Urologic Surgery, Washington University School of Medicine, St. Louis, MO, USA.; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA., Dodson KW; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.; Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, USA., Hultgren SJ; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA. hultgren@wustl.edu.; Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, USA. hultgren@wustl.edu., Earl AM; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, USA. aearl@broadinstitute.org.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2022 May; Vol. 7 (5), pp. 630-639. Date of Electronic Publication: 2022 May 02.
DOI: 10.1038/s41564-022-01107-x
Abstrakt: Recurrent urinary tract infections (rUTIs) are a major health burden worldwide, with history of infection being a significant risk factor. While the gut is a known reservoir for uropathogenic bacteria, the role of the microbiota in rUTI remains unclear. We conducted a year-long study of women with (n = 15) and without (n = 16) history of rUTI, from whom we collected urine, blood and monthly faecal samples for metagenomic and transcriptomic interrogation. During the study 24 UTIs were reported, with additional samples collected during and after infection. The gut microbiome of individuals with a history of rUTI was significantly depleted in microbial richness and butyrate-producing bacteria compared with controls, reminiscent of other inflammatory conditions. However, Escherichia coli gut and bladder populations were comparable between cohorts in both relative abundance and phylogroup. Transcriptional analysis of peripheral blood mononuclear cells revealed expression profiles indicative of differential systemic immunity between cohorts. Altogether, these results suggest that rUTI susceptibility is in part mediated through the gut-bladder axis, comprising gut dysbiosis and differential immune response to bacterial bladder colonization, manifesting in symptoms.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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