Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling.

Autor:	Taylor JAGE; Gut Microbes and Health Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK., Benwell CJ; Gut Microbes and Health Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK., Robinson SD; Gut Microbes and Health Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK. Stephen.Robinson@quadram.ac.uk.; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK. Stephen.Robinson@quadram.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2475, pp. 133-141.
DOI:	10.1007/978-1-0716-2217-9_9
Abstrakt:	The ability to study the role of specific genes in endothelial cell biology is made possible by our ability to modulate their expression through siRNA or knockout technologies. However, many in vitro protocols, particularly those of a biochemical nature, require large numbers of endothelial cells. These types of analyses are encumbered by the need to repeatedly produce and characterize primary endothelial cell cultures and can be greatly facilitated by the use of immortalized microvascular endothelial cells. However, we have found that the manipulation of gene expression in these cells is not always straight forward. Here we describe how we alter gene expression in polyoma middle T antigen immortalized microvascular endothelial cells isolated from wild-type and genetically modified mice to study the role of cell adhesion molecules in downstream assays. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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