Longitudinal analysis of T cell receptor repertoires reveals shared patterns of antigen-specific response to SARS-CoV-2 infection.

Autor: Gittelman RM; Adaptive Biotechnologies, Seattle, Washington, USA., Lavezzo E; Department of Molecular Medicine, University of Padova, Padua, Italy., Snyder TM; Adaptive Biotechnologies, Seattle, Washington, USA., Zahid HJ; Microsoft Research, Redmond, Washington, USA., Carty CL; Adaptive Biotechnologies, Seattle, Washington, USA., Elyanow R; Adaptive Biotechnologies, Seattle, Washington, USA., Dalai S; Adaptive Biotechnologies, Seattle, Washington, USA.; Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA., Kirsch I; Adaptive Biotechnologies, Seattle, Washington, USA., Baldo L; Adaptive Biotechnologies, Seattle, Washington, USA., Manuto L; Department of Molecular Medicine, University of Padova, Padua, Italy., Franchin E; Department of Molecular Medicine, University of Padova, Padua, Italy., Del Vecchio C; Department of Molecular Medicine, University of Padova, Padua, Italy., Pacenti M; Azienda Ospedale Padova, Microbiology and Virology Unit, Padua, Italy., Boldrin C; Azienda Ospedale Padova, Microbiology and Virology Unit, Padua, Italy., Cattai M; Azienda Ospedale Padova, Microbiology and Virology Unit, Padua, Italy., Saluzzo F; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele, Milan, Italy., Padoan A; Department of Medicine, University of Padova, Padua, Italy., Plebani M; Department of Medicine, University of Padova, Padua, Italy., Simeoni F; Center for Omics Sciences and., Bordini J; Division of Experimental Oncology, IRCCS Ospedale San Raffaele, Milan, Italy., Lorè NI; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele, Milan, Italy., Lazarević D; Center for Omics Sciences and., Cirillo DM; Division of Immunology, Transplantation and Infectious Disease, IRCCS Ospedale San Raffaele, Milan, Italy., Ghia P; Division of Experimental Oncology, IRCCS Ospedale San Raffaele, Milan, Italy.; Vita-Salute San Raffaele University, Milan, Italy., Toppo S; Department of Molecular Medicine, University of Padova, Padua, Italy.; CRIBI Biotech Center, University of Padova, Padua, Italy., Carlson JM; Microsoft Research, Redmond, Washington, USA., Robins HS; Adaptive Biotechnologies, Seattle, Washington, USA., Crisanti A; Department of Molecular Medicine, University of Padova, Padua, Italy.; Department of Life Sciences, Imperial College London, London, United Kingdom., Tonon G; Center for Omics Sciences and.; Division of Experimental Oncology, IRCCS Ospedale San Raffaele, Milan, Italy.; Vita-Salute San Raffaele University, Milan, Italy.
Jazyk: angličtina
Zdroj: JCI insight [JCI Insight] 2022 May 23; Vol. 7 (10). Date of Electronic Publication: 2022 May 23.
DOI: 10.1172/jci.insight.151849
Abstrakt: T cells play a prominent role in orchestrating the immune response to viral diseases, but their role in the clinical presentation and subsequent immunity to SARS-CoV-2 infection remains poorly understood. As part of a population-based survey of the municipality of Vo', Italy, conducted after the initial SARS-CoV-2 outbreak, we sampled the T cell receptor (TCR) repertoires of the population 2 months after the initial PCR survey and followed up positive cases 9 and 15 months later. At 2 months, we found that 97.0% (98 of 101) of cases had elevated levels of TCRs associated with SARS-CoV-2. T cell frequency (depth) was increased in individuals with more severe disease. Both depth and diversity (breadth) of the TCR repertoire were positively associated with neutralizing antibody titers, driven mostly by CD4+ T cells directed against spike protein. At the later time points, detection of these TCRs remained high, with 90.7% (78 of 96) and 86.2% (25 of 29) of individuals having detectable signal at 9 and 15 months, respectively. Forty-three individuals were vaccinated by month 15 and showed a significant increase in TCRs directed against spike protein. Taken together, these results demonstrate the central role of T cells in mounting an immune defense against SARS-CoV-2 that persists out to 15 months.
Databáze: MEDLINE
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