Novel insights into the potential mechanisms underlying carbendazim-induced hepatorenal toxicity in rats.

Autor: Ebedy YA; Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Elshazly MO; Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Hassan NH; Physiology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Ibrahim MA; Biochemistry Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Hassanen EI; Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Jazyk: angličtina
Zdroj: Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2022 Aug; Vol. 36 (8), pp. e23079. Date of Electronic Publication: 2022 Apr 19.
DOI: 10.1002/jbt.23079
Abstrakt: Carbendazim (CBZ) is a common environmental pollutant that can contaminate food and water and severely damage human health. Some studies revealed the adverse effect of CBZ on different organs, but its detailed toxicity mechanism has not been elucidated yet. Thus, the present study aims to clarify the mechanisms of CBZ-induced hepatorenal toxicity in rats. Therefore, we partitioned 40 male Wistar rats into four groups (n = 10): a negative control group and three treatment groups, which received 100, 300, and 600 mg/kg of CBZ. All rats received the treatment daily by oral gavage. We collected blood and organ samples (liver and kidney) at 14 and 28 days postdosing. CBZ caused extensive pathological alterations in both the liver and kidneys, such as cellular degeneration and necrosis accompanied by severe inflammatory reactions in a dose- and time-dependent manner. All the CBZ-treated groups displayed strong tumor necrosis factor-α and nuclear factor-κB (NF-κB) immunopositivity. Additionally, CBZ dose-dependently elevated the alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea, and creatinine serum levels and reduced the serum albumin levels. Furthermore, CBZ-induced apoptosis, as indicated by the observed Bax gene upregulation and Bcl-2 gene downregulation in both organs. All these changes may be related to oxidative stress, as indicated by the increase in malondialdehyde levels and the decrease in total antioxidant capacity. Our results demonstrate that CBZ-induced dose- and time-dependent hepatorenal damage through oxidative stress, which activated both the NF-κB signaling pathway and Bcl-based programmed cell death.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: