In vivo bio-distribution and acute toxicity evaluation of greenly synthesized ultra-small gold nanoparticles with different biological activities.

Autor: Aljohani FS; Chemistry Department, Faculty of Science, Taibah University, Medina, Saudi Arabia., Hamed MT; Botany & Microbiology Department, Faculty of Science, Alexandria University, P.O. Box 21568, Alexandria, Egypt., Bakr BA; Zoology Department, Faculty of Science, Alexandria University, P.O. Box 21568, Alexandria, Egypt., Shahin YH; Medical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, Egypt., Abu-Serie MM; Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt., Awaad AK; Biochemistry and Molecular Biology Department, Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria, Egypt., El-Kady H; Medical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, Egypt., Elwakil BH; Medical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, Egypt. bassma.hassan@pua.edu.eg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Apr 15; Vol. 12 (1), pp. 6269. Date of Electronic Publication: 2022 Apr 15.
DOI: 10.1038/s41598-022-10251-7
Abstrakt: Ultra-small gold nanoparticles (Au-NPs) "≤ 10 nm diameters" have potent biomedical applications. Hence, the present study aimed to greenly synthesize ultra-small gold nanoparticles using Egyptian propolis extract. Different biological activities, in vivo bio-distribution and acute toxicity study were assessed. Results revealed that, Egyptian propolis extract can successfully synthesize the highly pure and stable ultra-small Au-NPs with average diameter 7.8 nm. In vitro antimicrobial and antimycobacterial activities revealed the powerful effect of the prepared Au-NPs. Moreover, the cytotoxic effect on human cancer cell lines revealed the potent inhibition of the cancer cells' proliferation with high reactive oxygen species-mediated apoptosis induction. In vivo bio-distribution and acute toxicity studies were performed (10 and 100 mg/kg doses) in male albino rats. The ultra-small Au-NPs showed low or no toxicity upon using the Au-NPs low dose. The mean area accumulation (%) of the Au-NPs was higher in the liver, kidney, and brain tissues (4.41, 2.96, and 0.3 times, respectively) treated with high Au-NPs dosage compared to those treated with the low dose. Surprisingly, Au-NP accumulation in brain tissue was observed in the glial cells only. Accordingly, the low dose (10 mg/kg) of Au-NPs can be used safely in a variety of biomedical applications.
(© 2022. The Author(s).)
Databáze: MEDLINE
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