Multi-locus genome-wide association studies (ML-GWAS) reveal novel genomic regions associated with seedling and adult plant stage leaf rust resistance in bread wheat (Triticum aestivum L.).

Autor: Vikas VK; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Pradhan AK; ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India., Budhlakoti N; ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012, India. neeraj35669@gmail.com., Mishra DC; ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012, India., Chandra T; ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India., Bhardwaj SC; ICAR-Indian Institute of Wheat and Barley Research, Flowerdale, Shimla, Himachal Pradesh, 171002, India., Kumar S; ICAR-Indian Institute of Wheat and Barley Research, Flowerdale, Shimla, Himachal Pradesh, 171002, India., Sivasamy M; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Jayaprakash P; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Nisha R; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Shajitha P; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Peter J; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Geetha M; ICAR-Indian Agricultural Research Institute, Regional Station, Wellington, 643 231, India., Mir RR; Division of Genetics and Plant Breeding, Faculty of Agriculture (FoA), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, India., Singh K; ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India.; Genetic Resource Division, ICRISAT, Patancheru, Hyderabad, India., Kumar S; ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India. Sundeep.Kumar@icar.gov.in.
Jazyk: angličtina
Zdroj: Heredity [Heredity (Edinb)] 2022 Jun; Vol. 128 (6), pp. 434-449. Date of Electronic Publication: 2022 Apr 13.
DOI: 10.1038/s41437-022-00525-1
Abstrakt: Leaf rust is one of the important diseases limiting global wheat production and productivity. To identify quantitative trait nucleotides (QTNs) or genomic regions associated with seedling and adult plant leaf rust resistance, multilocus genome-wide association studies (ML-GWAS) were performed on a panel of 400 diverse wheat genotypes using 35 K single-nucleotide polymorphism (SNP) genotyping assays and trait data of leaf rust resistance. Association analyses using six multi-locus GWAS models revealed a set of 201 significantly associated QTNs for seedling and 65 QTNs for adult plant resistance (APR), explaining 1.98-31.72% of the phenotypic variation for leaf rust. Among these QTNs, 51 reliable QTNs for seedling and 15 QTNs for APR were consistently detected in at least two GWAS models and were considered reliable QTNs. Three genomic regions were pleiotropic, each controlling two to three pathotype-specific seedling resistances to leaf rust. We also identified candidate genes, such as leucine-rich repeat receptor-like (LRR) protein kinases, P-loop containing nucleoside triphosphate hydrolase and serine-threonine/tyrosine-protein kinases (STPK), which have a role in pathogen recognition and disease resistance linked to the significantly associated genomic regions. The QTNs identified in this study can prove useful in wheat molecular breeding programs aimed at enhancing resistance to leaf rust and developing next-generation leaf rust-resistant varieties.
(© 2022. The Author(s), under exclusive licence to The Genetics Society.)
Databáze: MEDLINE
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