Deciphering Chemical Mediators Regulating Specialized Metabolism in a Symbiotic Cyanobacterium.

Autor: Krumbholz J; Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24/25, 14476, Potsdam-Golm, Germany., Ishida K; Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Beutenbergstr. 11a, 07745, Jena, Germany., Baunach M; Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24/25, 14476, Potsdam-Golm, Germany., Teikari JE; Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24/25, 14476, Potsdam-Golm, Germany., Rose MM; Institute for Biology, Department of Plant Physiology, Leipzig University, Johannisallee 21-23, 04103, Leipzig, Germany., Sasso S; Institute for Biology, Department of Plant Physiology, Leipzig University, Johannisallee 21-23, 04103, Leipzig, Germany., Hertweck C; Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Beutenbergstr. 11a, 07745, Jena, Germany.; Institute of Microbiology, Faculty of Biological Sciences, Friedrich Schiller University Jena, 07743, Jena, Germany., Dittmann E; Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24/25, 14476, Potsdam-Golm, Germany.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2022 Jun 27; Vol. 61 (26), pp. e202204545. Date of Electronic Publication: 2022 May 09.
DOI: 10.1002/anie.202204545
Abstrakt: Genomes of cyanobacteria feature a variety of cryptic biosynthetic pathways for complex natural products, but the peculiarities limiting the discovery and exploitation of the metabolic dark matter are not well understood. Here we describe the discovery of two cell density-dependent chemical mediators, nostoclide and nostovalerolactone, in the symbiotic model strain Nostoc punctiforme, and demonstrate their pronounced impact on the regulation of specialized metabolism. Through transcriptional, bioinformatic and labeling studies we assigned two adjacent biosynthetic gene clusters to the biosynthesis of the two polyketide mediators. Our findings provide insight into the orchestration of specialized metabolite production and give lessons for the genomic mining and high-titer production of cyanobacterial bioactive compounds.
(© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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