Bovine mastitis in northeastern Brazil: Occurrence of emergent bacteria and their phenotypic and genotypic profile of antimicrobial resistance.

Autor: de Oliveira RP; Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE, Brazil. Electronic address: raylson.oliveira@hotmail.com.br., Aragão BB; Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE, Brazil., de Melo RPB; Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE, Brazil., da Silva DMS; Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE, Brazil., de Carvalho RG; Universidade Federal de São Paulo, Departamento de Biofísica, São Paulo, SP, Brazil., Juliano MA; Universidade Federal de São Paulo, Departamento de Biofísica, São Paulo, SP, Brazil., Farias MPO; Universidade Federal do Agreste Pernambucano, Departamento de Medicina Veterinária, Granhuns, PE, Brazil., de Lira NSC; Universidade Federal do Piauí, Departamento de Medicina Veterinária, Bom Jesus, PI, Brazil., Mota RA; Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, PE, Brazil.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2022 Jun; Vol. 85, pp. 101802. Date of Electronic Publication: 2022 Mar 29.
DOI: 10.1016/j.cimid.2022.101802
Abstrakt: The aim of this study was to identify emergent pathogens associated with bovine mastitis in northeastern Brazil and to characterize them for phenotypic and genotypic resistance to antimicrobials. A total of 321 milk samples from cows with subclinical mastitis were collected, and the isolates obtained in culture were identified using matrix-associated laser desorption-ionization - time of flight mass spectrometry. Phenotypic and genotypic antimicrobial resistance tests were performed. We identified 72 bacteria considered emergent in the study region: Enterococcus faecalis (26.3%; 19/72), Streptococcus agalactiae (22.2%; 16/72), Enterococcus faecium (20.0%; 15/72), Escherichia coli (6.9%; 5/72), 6.9% (5/72) Lactococcus garvieae (6.9%; 5/72), Acinetobacter baumannii (5.5%; 4/72), Bacillus subtilis (1.3%; 1/72), Kocuria marina (1.3%; 1/72), Macrococcus caseolyticus (1.3%; 1/72), Microbacterium resistens (1.3%; 1/72), Micrococcus luteus (1.3%; 1/72), Streptococcus dysgalactiae (1.3%; 1/72), Streptococcus hyovaginalis (1.3%; 1/72) and Streptococcus pluranimalium (1.3%; 1/72). The antibiogram revealed the following resistance profiles: ampicillin (77.7%; 56/72), cefoxitin (69.4%; 50/72), erythromycin (61.1%; 44/72), oxacillin (63.8%; 46/72), penicillin (79.1%; 57/72), tetracycline (63.8%; 46/72), gentamicin (25.0%; 18/72), and vancomycin (20.8%; 15/72). Of the isolates, 83.4% (60/72) showed multiple resistance to antimicrobials. The tetM gene was identified in 43.0% (31/72) of the isolates, followed by tetL (31.9%; 23/72), and blaZ (26.3%; 19/72). 83.4% (60/72) of the isolates presented a multiple antimicrobial resistance index higher than 0,2. Emergent bacteria with zoonotic and multiresistant potential occur in cows with mastitis in northeastern Brazil. It is necessary to monitor the occurrence of these and other bacteria in livestock environments and develop control strategies to prevent their spread.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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