| Autor: |
da Silva MC; Department of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil., Rocha DG; Federal Laboratory of Animal and Plant Health and Inspection of Minas Gerais, Ministry of Agriculture, Livestock and Food Supply of Brazil, Av. Rômulo Joviano, s/no, Olaria, Pedro Leopoldo, Minas Gerais 33250-220, Brazil., Pereira MPM; Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil., Lana MAG; Federal Laboratory of Animal and Plant Health and Inspection of Minas Gerais, Ministry of Agriculture, Livestock and Food Supply of Brazil, Av. Rômulo Joviano, s/no, Olaria, Pedro Leopoldo, Minas Gerais 33250-220, Brazil., Assis DCS; Veterinary School, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil., Faria AF; Department of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil. |
| Abstrakt: |
An in vivo study was performed in order to evaluate the depletion time of stanozolol and its main metabolites using naturally incurred urine sample collected after the administration of intramuscular injections in 12 steers. A stability study was also carried out to investigate the influence of the storage period and the freeze-thaw cycles. A fast parent drug metabolization was observed, because within 6 h after drug administration, the signal of the metabolite 16β-hydroxystanozolol was predominant. After the second drug administration, a detection window of 17 days was obtained. The stability was studied using ANOVA, in which a storage condition of -20 °C proved stable during 240 days, which was also confirmed after 5 freeze-thaw cycles. |
