Serum Levels of Growth-Associated Protein-43 and Neurotrophin-3 in Childhood Epilepsy and Their Relation to Zinc Levels.

Autor: Bakri AH; Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt., Hassan MH; Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, 83523, Egypt. mohammedhosnyhassaan@med.svu.edu.eg., Ahmed AE; Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt., Alotaibi G; Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Al-Dawadmi Campus, Shaqra, Saudi Arabia., Halim PR; Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt., Abdallah AAM; Medical Biochemistry Department, Faculty of Medicine, Sohag University, Sohag, Egypt., Rashwan NI; Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2023 Feb; Vol. 201 (2), pp. 689-697. Date of Electronic Publication: 2022 Mar 29.
DOI: 10.1007/s12011-022-03213-7
Abstrakt: Background: Epilepsy is one of the most common neurological disorders, and it places a significant economic strain on the healthcare system around the world. Although the exact mechanism of epilepsy has yet to be illustrated, various pathogenic cascades involving neurotransmitters and trace elements have been reported. We aimed to investigate the serum levels of growth-associated protein-43 (GAP-43) and neurotrophin-3 (NT-3) among cohort of Egyptian children with epilepsy and correlate these biomarkers with their zinc levels.
Methods: This case-control study included 50 pediatric patients with epilepsy who were comparable with 50 controls. Neurological assessment and electroencephalogram (EEG) were done to all included children. Biochemical measurements of serum GAP-43 and NT-3 using enzyme linked immunosorbent assays (ELISA), and total antioxidant capacity (TAC) and zinc using colorimetric assays, were performed to all participants.
Results: There was significantly frequent positive parental consanguinity among cases with significantly frequent generalized onset seizures (94%) than simple partial seizure (6%). There were significantly lower serum GAP-43 and zinc levels with significantly higher TAC among cases vs. the controls, p˂0.05 for all. There was no significant difference in the serum levels of NT-3 among epileptic children vs. the controls, p = 0.269. Serum Zn was positively correlated with GAP-43 level among epileptic children (r = 0.381, p = 0.006). Serum GAP-43 in diagnosing childhood epilepsy at cut-off point ≤ 0.6 ng/mL showed 78% sensitivity, 62% specificity, positive predictive value (PPV) = 50.6%, negative predictive value (NPP) = 84.9% with AUC = 0.574.
Conclusion: GAP-43 can be considered a sensitive good negative biomarker in childhood epilepsy which correlated positively with the zinc status.
(© 2022. The Author(s).)
Databáze: MEDLINE
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