SUMO-mediated recruitment allows timely function of the Yen1 nuclease in mitotic cells.

Autor: Dorison H; Université Paris-Saclay-UMR9019 CNRS-Gustave Roussy, Villejuif, France., Talhaoui I; Université Paris-Saclay-UMR9019 CNRS-Gustave Roussy, Villejuif, France., Mazón G; Université Paris-Saclay-UMR9019 CNRS-Gustave Roussy, Villejuif, France.; Inserm-Institut National de la Santé et de la Recherche Médicale, Paris, France.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2022 Mar 25; Vol. 18 (3), pp. e1009860. Date of Electronic Publication: 2022 Mar 25 (Print Publication: 2022).
DOI: 10.1371/journal.pgen.1009860
Abstrakt: The post-translational modification of DNA damage response proteins with SUMO is an important mechanism to orchestrate a timely and orderly recruitment of repair factors to damage sites. After DNA replication stress and double-strand break formation, a number of repair factors are SUMOylated and interact with other SUMOylated factors, including the Yen1 nuclease. Yen1 plays a critical role in ensuring genome stability and unperturbed chromosome segregation by removing covalently linked DNA intermediates between sister chromatids that are formed by homologous recombination. Here we show how this important role of Yen1 depends on interactions mediated by non-covalent binding to SUMOylated partners. Mutations in the motifs that allow SUMO-mediated recruitment of Yen1 impair its ability to resolve DNA intermediates and result in chromosome mis-segregation and increased genome instability.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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