Longitudinal morphological and functional characterization of human heart organoids using optical coherence tomography.

Autor: Ming Y; Department of Biomedical Engineering, Washington University in Saint Louis, USA., Hao S; Department of Electrical & Systems Engineering, Washington University in Saint Louis, USA., Wang F; Department of Biomedical Engineering, Washington University in Saint Louis, USA., Lewis-Israeli YR; Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, USA; Department of Biomedical Engineering, College of Engineering, Michigan State University, USA., Volmert BD; Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, USA; Department of Biomedical Engineering, College of Engineering, Michigan State University, USA., Xu Z; Department of Biomedical Engineering, Washington University in Saint Louis, USA., Goestenkors A; Department of Biomedical Engineering, Washington University in Saint Louis, USA., Aguirre A; Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, USA; Department of Biomedical Engineering, College of Engineering, Michigan State University, USA., Zhou C; Department of Biomedical Engineering, Washington University in Saint Louis, USA. Electronic address: chaozhou@wustl.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2022 Jul 01; Vol. 207, pp. 114136. Date of Electronic Publication: 2022 Mar 09.
DOI: 10.1016/j.bios.2022.114136
Abstrakt: Organoids play an increasingly important role as in vitro models for studying organ development, disease mechanisms, and drug discovery. Organoids are self-organizing, organ-like three-dimensional (3D) cell cultures developing organ-specific cell types and functions. Recently, three groups independently developed self-assembling human heart organoids (hHOs) from human pluripotent stem cells (hPSCs). In this study, we utilized a customized spectral-domain optical coherence tomography (SD-OCT) system to characterize the growth of hHOs. Development of chamber structures and beating patterns of the hHOs were observed via OCT and calcium imaging. We demonstrated the capability of OCT to produce 3D images in a fast, label-free, and non-destructive manner. The hHOs formed cavities of various sizes, and complex interconnections were observed as early as on day 4 of differentiation. The hHOs models and the OCT imaging system showed promising insights as an in vitro platform for investigating heart development and disease mechanisms.
(Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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