| Autor: |
Laksmitawati DR; Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia., Widowati W; Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia., Noverina R; Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung, West Java, Indonesia., Ayuningtyas W; Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung, West Java, Indonesia., Kurniawan D; Animal and Stem Cells Laboratory, PT Bio Farma (Persero), Bandung, West Java, Indonesia., Kusuma HSW; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia., Afifah E; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia., Rinendyaputri R; National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia., Rilianawati R; Agency for the Assesment and Application of Technology, Ministry of Research and Technology, Serpong, Banten, Indonesia., Faried A; Department of Neurosurgery, Faculty of Medicine, Padjadjaran University, Bandung, West Java, Indonesia., Susilarini NK; National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia. |
| Abstrakt: |
BACKGROUND Inflammation is the body's first response to an illness that causes irritation or infection. Inflammation is tightly correlated with aging, which is a progressive degenerative process. Conditioned medium (CM) from adipose tissue-derived mesenchymal stem cells (CM-ATMSCs) has been shown to stimulate collagen synthesis and dermal fibroblast migration, as well as reduce wrinkles and improve wound healing. This study aimed to observe the production of inflammatory modulators - interleukin (IL)-1alpha, IL-6, IL-10, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) - in CM-ATMSCs treated with fresh frozen plasma (FFP) at passages 3 (P3), 7, 11, and 15. MATERIAL AND METHODS ATMSCs P3 were obtained from liposuction of female donors, and the CM from ATMSCs was collected. Measurement of these cytokines was performed with ELISA. RESULTS At many passages, IL-6, a proinflammatory modulator, was discovered to be the most powerful modulator among FFP- and non-FFP-treated cells. However, CM-ATMSCs treated with FFP and in the late passage have significant differences (P<0.05) compared to non-FFP treatments and in other passages in their effects on secretion of inflammatory modulators. CONCLUSIONS In conclusion, CM-ATMSC has the potential to secrete proinflammatory modulators. |
