Gas chromatography combined with flame-induced atmospheric pressure chemical ionization mass spectrometry for the analysis of fatty acid methyl esters and saturated hydrocarbons.

Autor: Cheng SC; Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan, 741, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan. Electronic address: sychyi@gate.sinica.edu.tw., Lin HJ; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Lee CY; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Huang MZ; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Shiea J; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: jetea@mail.nsysu.edu.tw.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2022 Apr 01; Vol. 1200, pp. 339611. Date of Electronic Publication: 2022 Feb 16.
DOI: 10.1016/j.aca.2022.339611
Abstrakt: A simple flame-induced atmospheric pressure chemical ionization (FAPCI) source was developed to couple a gas chromatograph (GC) with a mass spectrometer (MS). The interface consisted of a heated transfer line and a high voltage-free ambient FAPCI source. Nitrogen gas flowing through the heated transfer line was utilized to deliver the analytes eluted from a GC column to the ionization region. A micro oxyacetylene flame was positioned under the exit of heated transfer line, which generated primary charged species in the ionization region. Since the temperature at the ionization region was below 200 °C, the analytes were not thermally decomposed. Protonated analytes were formed by reacting the analytes with flame-induced charged species through ion-molecule reactions (IMRs). The simple GC-FAPCI/MS was used to characterize a series of fatty acid methyl esters (FAMEs) and long-chain normal alkanes, which showed protonated FAME and oxidized n-alkane ions on the mass spectra. The limits of detection (LODs) for C15:0 to C25:0 FAMEs were 1-2.5 pg. A calibration curve ranging from 2.5 to 500 pg, with a R 2 value of 0.9821, was obtained.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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