Withania somnifera Chemical Constituents' In Vitro Antioxidant Potential and Their Response on Spermatozoa Parameters.

Autor: Munir N; Department of Basic Medical Sciences, School of Health Sciences, University of Management and Technology, Lahore, Pakistan.; Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan., Mahmood Z; Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan., Shahid M; Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan., Afzal MN; Department of Basic Medical Sciences, School of Health Sciences, University of Management and Technology, Lahore, Pakistan., Jahangir M; Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan., Ali Shah SM; Department of Eastern Medicine and Surgery, Government College University Faisalabad, Faisalabad, Pakistan., Tahir IM; College of Allied Health Professionals, Government College University Faisalabad, Faisalabad, Pakistan., Riaz M; Department of Allied Health Sciences, Sargodha Medical College, University of Sargodha, Sargodha, Pakistan., Hussain S; Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan., Akram M; Department of Eastern Medicine and Surgery, Government College University Faisalabad, Faisalabad, Pakistan., Yousaf F; Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Jazyk: angličtina
Zdroj: Dose-response : a publication of International Hormesis Society [Dose Response] 2022 Mar 01; Vol. 20 (1), pp. 15593258221074936. Date of Electronic Publication: 2022 Mar 01 (Print Publication: 2022).
DOI: 10.1177/15593258221074936
Abstrakt: Background: Phytomedicine is becoming more acceptable as an alternative medicinal approach in the modern era. Objectives: The current study examined the antioxidant capacity and in vitro response of phytochemical constituents of  Withania somnifera  (Ashwagandha) on standard parameters of healthy volunteer semen. Methods: The phytochemicals and their pharmacological response in a hydroethanolic (30:70 v/v) extract of  W .  somnifera  roots were determined using standard protocols. Results: The constituents included flavonoids, phenolic acids, alkaloids, and terpenoids were reported. High-performance liquid chromatography and Fourier-transform infrared spectroscopy determined a diverse array of biologically active chemical constituents in the extract. The extract of  W. somnifera  exhibits substantial antioxidant properties, including total antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl inhibition, H 2 O 2  scavenging, and Fe 3+  reducing potential ( P < .05). The analysis of essential natural minerals explored adequate levels determined using atomic absorption spectrophotometer. Cytotoxic studies revealed significant thrombolytic, RBC membrane stabilization, and DNA damage protection activity ( P < .05) while remaining non-mutagenic against  Salmonella typhi  TA98 and TA100. The best protective response of  W. somnifera  extract on human semen parameters (n = 30), such as total motility, progressive motility, and viability, demonstrated a significant ( P  < .05) improvement, particularly at the dose of 25 μg/mL and 50 μg/mL. Conclusion: The study concludes that  W. somnifera  possesses favorable in vitro characteristics that could aid in the preservation of sperm during intrauterine insemination and in vitro fertilization.
Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(© The Author(s) 2022.)
Databáze: MEDLINE
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