Horizontal mtDNA transfer between cells is common during mouse development.

Autor:	Marti Gutierrez N; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Mikhalchenko A; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Ma H; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Koski A; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Li Y; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Van Dyken C; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Tippner-Hedges R; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Yoon D; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Liang D; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Hayama T; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Battaglia D; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA., Kang E; Stem Cell Center & Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, South Korea., Lee Y; Stem Cell Center & Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, South Korea., Barnes AP; Knight Cardiovascular Institute, Department of Medicine, Oregon Health & Science University, Portland, OR 97239, USA., Amato P; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.; Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA., Mitalipov S; Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2022 Feb 10; Vol. 25 (3), pp. 103901. Date of Electronic Publication: 2022 Feb 10 (Print Publication: 2022).
DOI:	10.1016/j.isci.2022.103901
Abstrakt:	Cells transmit their genomes vertically to daughter cells during cell divisions. Here, we demonstrate the occurrence and extent of horizontal mitochondrial (mt)DNA acquisition between cells that are not in a parent-offspring relationship. Extensive single-cell sequencing from various tissues and organs of adult chimeric mice composed of cells carrying distinct mtDNA haplotypes showed that a substantial fraction of individual cardiomyocytes, neurons, glia, intestinal, and spleen cells captured donor mtDNA at high levels. In addition, chimeras composed of cells with wild-type and mutant mtDNA exhibited increased trafficking of wild-type mtDNA to mutant cells, suggesting that horizontal mtDNA transfer may be a compensatory mechanism to restore compromised mitochondrial function. These findings establish the groundwork for further investigations to identify mtDNA donor cells and mechanisms of transfer that could be critical to the development of novel gene therapies. Competing Interests: The authors declare no competing interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu