Development of Avocado Reference Material for Pesticide Residue Analysis.

Autor: Morales LV; Instituto Nacional de Metrología de Colombia, Grupo de Investigación en Metrología Química y Bioanálisis, Avenida Carrera 50, No. 26-55, 11132 Bogotá D.C., Colombia.; Universidad Nacional de Colombia, Grupo de Investigación en Metrología Química, LEA, Avenida Carrera 30 45-03, 11132 Bogotá D.C., Colombia., Salinas AS; Instituto Nacional de Metrología de Colombia, Grupo de Investigación en Metrología Química y Bioanálisis, Avenida Carrera 50, No. 26-55, 11132 Bogotá D.C., Colombia., González IA; Instituto Nacional de Metrología de Colombia, Grupo de Investigación en Metrología Química y Bioanálisis, Avenida Carrera 50, No. 26-55, 11132 Bogotá D.C., Colombia., Holguin K; Instituto Nacional de Metrología de Colombia, Grupo de Investigación en Metrología Química y Bioanálisis, Avenida Carrera 50, No. 26-55, 11132 Bogotá D.C., Colombia., Sinuco DC; Universidad Nacional de Colombia, Grupo de Investigación en Metrología Química, LEA, Avenida Carrera 30 45-03, 11132 Bogotá D.C., Colombia., Díaz-Galiano FJ; European Union Reference Laboratory for Pesticide Residues in Fruits and Vegetables, University of Almería, 04120 Almería, Spain., Ahumada DA; Instituto Nacional de Metrología de Colombia, Grupo de Investigación en Metrología Química y Bioanálisis, Avenida Carrera 50, No. 26-55, 11132 Bogotá D.C., Colombia., Fernández-Alba AR; European Union Reference Laboratory for Pesticide Residues in Fruits and Vegetables, University of Almería, 04120 Almería, Spain.
Jazyk: angličtina
Zdroj: Journal of AOAC International [J AOAC Int] 2022 Jun 29; Vol. 105 (4), pp. 1051-1059.
DOI: 10.1093/jaoacint/qsac034
Abstrakt: Background: Pesticide quantitation in foods relies on the availability of matrix reference materials, which, however, are scarce because of the general instability of pesticides. In particular, no avocado reference material has been developed.
Objective: This research aimed to develop a reference material to support the determination of pesticide residues in avocado.
Method: Avocado was spiked with 11 selected pesticides at levels of 0.01-0.35 mg/kg. The production process included the assessment of between-unit heterogeneity, stability during dispatch, and best storage conditions according to ISO 17034. Reference values were estimated through an interlaboratory comparison study involving laboratories of demonstrated competence and adhering to ISO/IEC 17025. The corresponding expanded uncertainties were calculated as 4-19% in compliance with the Guide to the Expression of Uncertainty in Measurement.
Results: The reference material was sufficiently homogeneous and stable at 4°C during the entire study period (365 days) for most of the pesticides, and at 40°C during 10 days, but it was unstable at 50 and 60°C during 10 days. Four pesticides showed downward trends; however, this behavior was considered in the uncertainty budget. As this material complied with all requirements of proficiency testing, it was used in an interlaboratory proficiency test designed to investigate analytical performance and assist laboratories in improving the quality of measurement results.
Conclusions: The presented material can be used for the development of novel analytical methods or in-house reference materials and adds to the scarce supply of reference materials for the determination of pesticides in vegetable matrixes with high oil contents and intermediate water contents.
Highlights: A novel reference material has been developed for the reliable and accurate quantitation of multiple pesticides in avocado, allowing researchers to avoid the preparation of in-house reference materials for this purpose.
(© The Author(s) 2022. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze: MEDLINE
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