| Autor: |
Elsharkawy MM; Agricultural Botany Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt., Kuno S; Laboratory of Plant Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu City 501-1193, Japan., Hyakumachi M; Laboratory of Plant Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu City 501-1193, Japan., Mostafa YS; Department of Biology, College of Science, King Khalid University, Abha 9004, Saudi Arabia., Alamri SA; Department of Biology, College of Science, King Khalid University, Abha 9004, Saudi Arabia., Alrumman SA; Department of Biology, College of Science, King Khalid University, Abha 9004, Saudi Arabia. |
| Abstrakt: |
The soil-borne pathogens Rhizoctonia solani and Sclerotium rolfsii have emerged as major pathogens of radish ( Raphanus sativus ) worldwide. The induction of soil suppressive of radish root rot disease was evaluated in soil repeatedly inoculated with R. solani , nonpathogenic binucleate Rhizoctonia sp. AG-A W1 (BNR) and S. rolfsii . The repeated inoculations of soil with R. solani and BNR significantly suppressed the disease severity of R. solani and S. rolfsii compared to the control. In contrast, the repeated inoculation of soil with S. rolfsii significantly suppressed only the pathogen, S. rolfsii . The community structure was examined using PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) method. The bands of Trichoderma sp. were observed in the first, second and third inoculations of the soil with BNR. Similarly, bands of Trichoderma sp. were observed in the second and third inoculations of the soil with S. rolfsii and R. solani . Compared to the control, disease severity was significantly reduced in the soil repeatedly inoculated with S. rolfsii and R. solani . In conclusion, Trichoderma species were accumulated in specific patterns depending on the applied fungal inoculum in the suppressive soil. |
