| Autor: |
Ben Sassi-Zaidy Y; Laboratory of Diversity, Management and Conservation of Biological Systems, LR18ES06, Faculty of Sciences of Tunis, University of Tunis El Manar, El Manar II Tunis 2092, Tunisia.; Department of Agronomy, Animal, Food, Natural Resources and Environment, University of Padova, 35020 Legnaro Padova, Italy.; Laboratory of Animal Genetic and Feed Resources Research, Department of Animal Science, Institut National Agronomique de Tunis, University of Carthage, Tunis-Mahrajène Tunis 1082, Tunisia., Mohamed-Brahmi A; Laboratory of Agricultural Production Systems Sustainability in the North Western Region of Tunisia, Department of Animal Production, Ecole Supérieure d'Agriculture du Kef Boulifa, University of Jendouba, Le Kef 7119, Tunisia., Nouairia G; Department of Agronomy, Animal, Food, Natural Resources and Environment, University of Padova, 35020 Legnaro Padova, Italy.; Department of Biochemistry and Biophysics, Stockholm University, SE-10691 Stockholm, Sweden., Charfi-Cheikhrouha F; Laboratory of Diversity, Management and Conservation of Biological Systems, LR18ES06, Faculty of Sciences of Tunis, University of Tunis El Manar, El Manar II Tunis 2092, Tunisia., Djemali M; Laboratory of Animal Genetic and Feed Resources Research, Department of Animal Science, Institut National Agronomique de Tunis, University of Carthage, Tunis-Mahrajène Tunis 1082, Tunisia., Cassandro M; Department of Agronomy, Animal, Food, Natural Resources and Environment, University of Padova, 35020 Legnaro Padova, Italy. |
| Abstrakt: |
This study analyzed the genetic variability, inbreeding and population structure of the Tunisian-North African dairy sheep breed, the Sicilo-Sarde (SS), created by crossing the Sarda and Comisana dairy breeds. The level of variability in the SS, considered as an endangered breed after a dramatic decrease, was assessed using 17 microsatellite markers by analyzing the two breed populations sampled from their respective cradles: SS of Beja (SS B , n = 27) and SS of Mateur (SS M , n = 25). High levels of genetic diversity in SS were revealed, with a total of 212 alleles, a high mean number of alleles (12.47 ± 4.17) and a high average polymorphism information content (PIC) (0.81 ± 0.10). The observed heterozygosity was considerable in SS B and SS M (0.795 and 0.785, respectively). The inbreeding level measured by the population inbreeding coefficient F IS is higher in the SS M population (0.121) than in the SS B population (0.090). The higher genetic diversity level detected in SS B reflected the effect of new Italian Sarda genes introduced by intra-uterine artificial insemination recently practiced in this population. The Wilcoxon test and the mode-shift distribution indicated that the SS breed is a non-bottlenecked population. The structural analysis reflected the historical miscegenation practiced during the breed creation and highlighted further ancient miscegenation, which could date back to the first waves of sheep introduction to the western Mediterranean region. Microsatellite markers were successfully applied in the assessment of the genetic variability of SS and should be used in monitoring this variability during the application of conservation strategies. |
