Comparison of genome architecture at two stages of male germline cell differentiation in Drosophila.

Autor:	Ilyin AA; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow 123182, Russia., Kononkova AD; Skolkovo Institute of Science and Technology, Skolkovo 143026, Russia.; A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow 127051, Russia., Golova AV; Skolkovo Institute of Science and Technology, Skolkovo 143026, Russia., Shloma VV; Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia., Olenkina OM; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow 123182, Russia., Nenasheva VV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow 123182, Russia., Abramov YA; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow 123182, Russia., Kotov AA; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow 123182, Russia., Maksimov DA; Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia., Laktionov PP; Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.; Novosibirsk State University, Novosibirsk 630090, Russia., Pindyurin AV; Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia., Galitsyna AA; Skolkovo Institute of Science and Technology, Skolkovo 143026, Russia., Ulianov SV; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow 119992, Russia., Khrameeva EE; Skolkovo Institute of Science and Technology, Skolkovo 143026, Russia., Gelfand MS; Skolkovo Institute of Science and Technology, Skolkovo 143026, Russia.; A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow 127051, Russia., Belyakin SN; Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.; Novosibirsk State University, Novosibirsk 630090, Russia., Razin SV; Institute of Gene Biology, Russian Academy of Sciences, Moscow119334, Russia.; Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow 119992, Russia., Shevelyov YY; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow 123182, Russia.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2022 Apr 08; Vol. 50 (6), pp. 3203-3225.
DOI:	10.1093/nar/gkac109
Abstrakt:	Eukaryotic chromosomes are spatially segregated into topologically associating domains (TADs). Some TADs are attached to the nuclear lamina (NL) through lamina-associated domains (LADs). Here, we identified LADs and TADs at two stages of Drosophila spermatogenesis - in bamΔ86 mutant testes which is the commonly used model of spermatogonia (SpG) and in larval testes mainly filled with spermatocytes (SpCs). We found that initiation of SpC-specific transcription correlates with promoters' detachment from the NL and with local spatial insulation of adjacent regions. However, this insulation does not result in the partitioning of inactive TADs into sub-TADs. We also revealed an increased contact frequency between SpC-specific genes in SpCs implying their de novo gathering into transcription factories. In addition, we uncovered the specific X chromosome organization in the male germline. In SpG and SpCs, a single X chromosome is stronger associated with the NL than autosomes. Nevertheless, active chromatin regions in the X chromosome interact with each other more frequently than in autosomes. Moreover, despite the absence of dosage compensation complex in the male germline, randomly inserted SpG-specific reporter is expressed higher in the X chromosome than in autosomes, thus evidencing that non-canonical dosage compensation operates in SpG. (© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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