| Autor: |
André CM; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg.; The New Zealand Institute for Plant and Food Research Limited (PFR), Private Bag 92169, Auckland 1142, New Zealand., Guerriero G; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg., Lateur M; Walloon Agricultural Research Centre, Rue de Liroux, B-5030 Gembloux, Belgium., Charton S; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg., Leclercq CC; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg., Renaut J; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg., Hausman JF; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg., Legay S; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 5 Rue Bommel, Hautcharage, L-4940 Luxembourg, Luxembourg. |
| Abstrakt: |
Apple russeting develops on the fruit surface when skin integrity has been lost. It induces a modification of fruit wax composition, including its triterpene profile. In the present work, we studied two closely related apple varieties, 'Reinette grise du Canada' and 'Reinette blanche du Canada', which display russeted and non-russeted skin phenotypes, respectively, during fruit development. To better understand the molecular events associated with russeting and the differential triterpene composition, metabolomics data were generated using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) and combined with proteomic and transcriptomic data. Our results indicated lower expression of genes linked to cuticle biosynthesis (cutin and wax) in russet apple throughout fruit development, along with an alteration of the specialized metabolism pathways, including triterpene and phenylpropanoid. We identified a lipid transfer protein (LTP3) as a novel player in cuticle formation, possibly involved in the transport of both cutin and wax components in apple skin. Metabolomic data highlighted for the first time a large diversity of triterpene-hydroxycinnamates in russeted tissues, accumulation of which was highly correlated with suberin-related genes, including some enzymes belonging to the BAHD (HXXXD-motif) acyltransferase family. Overall, this study increases our understanding about the crosstalk between triterpene and suberin pathways. |
