Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites.

Autor: Liffner B; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia., Balbin JM; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia., Shami GJ; Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, 3010, Australia., Siddiqui G; Dept Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia., Strauss J; Centre for Structural Systems Biology, 22607, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.; Biology Department, University of Hamburg, 20146, Hamburg, Germany.; GEOMAR Helmholtz Centre for Ocean Research Kiel, 24105, Kiel, Germany., Frölich S; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia., Heinemann GK; Experimental Therapeutics Laboratory, Clinical and Health Science Unit, University of South Australia, Adelaide, SA, 5000, Australia., Edwards EM; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia., Alder A; Centre for Structural Systems Biology, 22607, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.; Biology Department, University of Hamburg, 20146, Hamburg, Germany., Wichers JS; Centre for Structural Systems Biology, 22607, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany., Creek DJ; Dept Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia., Tilley L; Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, 3010, Australia., Dixon MWA; Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, 3010, Australia.; Department of Infectious Diseases, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, 3000, Australia.; Division of Infectious Diseases and Immune Defence, Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Australia., Gilberger TW; Centre for Structural Systems Biology, 22607, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.; Biology Department, University of Hamburg, 20146, Hamburg, Germany., Wilson DW; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, 5005, Australia. danny.wilson@adelaide.edu.au.; Institute for Photonics and Advanced Sensing, University of Adelaide, Adelaide, 5005, Australia. danny.wilson@adelaide.edu.au.; Burnet Institute, 85 Commercial Road, Melbourne, VIC, 3004, Australia. danny.wilson@adelaide.edu.au.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2022 Feb 09; Vol. 5 (1), pp. 121. Date of Electronic Publication: 2022 Feb 09.
DOI: 10.1038/s42003-022-03020-9
Abstrakt: Merozoite invasion of host red blood cells (RBCs) is essential for survival of the human malaria parasite Plasmodium falciparum. Proteins involved with RBC binding and invasion are secreted from dual-club shaped organelles at the apical tip of the merozoite called the rhoptries. Here we characterise P. falciparum Cytosolically Exposed Rhoptry Leaflet Interacting protein 2 (PfCERLI2), as a rhoptry bulb protein that is essential for merozoite invasion. Phylogenetic analyses show that cerli2 arose through an ancestral gene duplication of cerli1. We show that PfCERLI2 is essential for blood-stage growth and localises to the cytosolic face of the rhoptry bulb. Inducible knockdown of PfCERLI2 led to a proportion of merozoites failing to invade and was associated with elongation of the rhoptry organelle during merozoite development and inhibition of rhoptry antigen processing. These findings identify PfCERLI2 as a protein that has key roles in rhoptry biology during merozoite invasion.
(© 2022. The Author(s).)
Databáze: MEDLINE
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