Variability in the composition of porcine mucosal heparan sulfates.

Autor: Sargison L; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Liam.Sargison@vuw.ac.nz., Smith RAA; Institute of Molecular and Cell Biology (IMCB), Glycotherapeutics Group, Agency for Science, Technology and Research (A*STAR), A*STAR, 138673, Singapore. Electronic address: Raymond_Alexander_Alfred_Smith@imcb.a-star.edu.sg., Carnachan SM; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Susie.Carnachan@vuw.ac.nz., Daines AM; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Alison.Daines@vuw.ac.nz., Brackovic A; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Amira.Brackovic@vuw.ac.nz., Kidgell JT; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Joel.Kidgell@vuw.ac.nz., Nurcombe V; Institute of Molecular and Cell Biology (IMCB), Glycotherapeutics Group, Agency for Science, Technology and Research (A*STAR), A*STAR, 138673, Singapore., Cool SM; Institute of Molecular and Cell Biology (IMCB), Glycotherapeutics Group, Agency for Science, Technology and Research (A*STAR), A*STAR, 138673, Singapore. Electronic address: Simon_Cool@imcb.a-star.edu.sg., Sims IM; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Ian.Sims@vuw.ac.nz., Hinkley SFR; The Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Lower Hutt 5040, New Zealand. Electronic address: Simon.Hinkley@vuw.ac.nz.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2022 Apr 15; Vol. 282, pp. 119081. Date of Electronic Publication: 2022 Jan 04.
DOI: 10.1016/j.carbpol.2021.119081
Abstrakt: Commercial porcine intestinal mucosal heparan sulfate (HS) is a valuable material for research into its biological functions. As it is usually produced as a side-stream of pharmaceutical heparin manufacture, its chemical composition may vary from batch to batch. We analysed the composition and structure of nine batches of HS from the same manufacturer. Statistical analysis of the disaccharide compositions placed these batches in three categories: group A had high GlcNAc and GlcNS, and low GlcN typical of HS; group B had high GlcN and GlcNS, and low GlcNAc; group C had high di- and trisulfated, and low unsulfated and monosulfated disaccharide repeats. These batches could be placed in the same categories based on their 1 H NMR spectra and molecular weights. Anticoagulant and growth factor binding activities of these HS batches did not fit within these same groups but were related to the proportions of more highly sulfated disaccharide repeats.
(Copyright © 2021. Published by Elsevier Ltd.)
Databáze: MEDLINE
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