Neuroprotective Effect of Riboflavin Producing Lactic Acid Bacteria in Parkinsonian Models.

Autor: Perez Visñuk D; Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina., Teran MDM; Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina., Savoy de Giori G; Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina.; Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina., LeBlanc JG; Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina. leblanc@cerela.org.ar., de Moreno de LeBlanc A; Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina. demoreno@cerela.org.ar.
Jazyk: angličtina
Zdroj: Neurochemical research [Neurochem Res] 2022 May; Vol. 47 (5), pp. 1269-1279. Date of Electronic Publication: 2022 Feb 03.
DOI: 10.1007/s11064-021-03520-w
Abstrakt: Oxidative stress and inflammatory processes might contribute to the cascade of events leading Parkinson disease (PD); and vitamins such as riboflavin can exert protection on vulnerable neurons in neurodegenerative conditions. Previously, it was demonstrated that a mixture of lactic acid bacteria (including a riboflavin-producing strain) improved motor skills in a parkinsonian model. The aim of the present work was to investigate the neuroprotective potential of Lactiplantibacillus (L.) plantarum CRL2130, a riboflavin-producing strain in PD models. In vitro, N2a differentiated neurons were exposed the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) and treated with intracellular bacterial extracts or commercial riboflavin. In vivo, adult male C57BL/6 mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and probenecid, and received orally L. plantarum CRL2130, L. plantarum CRL725 (parent strain that produces low levels of riboflavin) or commercial vitamin. Results showed that when N2a cells were incubated with intracellular extract from L. plantarum CRL2130 maintained the viability, and significantly decreased the release of IL-6 and the formation of reactive oxygen species (ROS), all affected by MPP+. In vivo, the administration of L. plantarum CRL2130 attenuated motor deficits and prevented dopaminergic neuronal death. Decrease of pro-inflammatory cytokines and increase of IL-10 in both serum and brain were observed in samples from mice that received L. plantarum CRL2130 compared to MPTP control group (without treatment). In addition, these beneficial effects were similar or improved when compared with animals that received commercial riboflavin. In conclusion, L. plantarum CRL2130 showed a neuroprotective effect in both PD models through anti-oxidant/anti-inflammatory mechanisms.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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