Evaluation of the Acute Effects of Arsenic on Adults of the Neotropical Native Fish Cnesterodon decemmaculatus Using a Set of Biomarkers.

Autor: González Núñez AA; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina.; Instituto de Ecología y Desarrollo Sustentable (Universidad Nacional de Luján, Consejo Nacional de Investigaciones Científicas y Técnicas), Luján, Buenos Aires, Argentina., Ferro JP; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina.; Instituto de Ecología y Desarrollo Sustentable (Universidad Nacional de Luján, Consejo Nacional de Investigaciones Científicas y Técnicas), Luján, Buenos Aires, Argentina., Campos LB; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina.; Instituto de Ecología y Desarrollo Sustentable (Universidad Nacional de Luján, Consejo Nacional de Investigaciones Científicas y Técnicas), Luján, Buenos Aires, Argentina., Eissa BL; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina.; Instituto de Ecología y Desarrollo Sustentable (Universidad Nacional de Luján, Consejo Nacional de Investigaciones Científicas y Técnicas), Luján, Buenos Aires, Argentina., Mastrángelo MM; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina.; Instituto de Ecología y Desarrollo Sustentable (Universidad Nacional de Luján, Consejo Nacional de Investigaciones Científicas y Técnicas), Luján, Buenos Aires, Argentina., Ferrari L; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina., Ossana NA; Programa de Ecofisiología Aplicada, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina.; Instituto de Ecología y Desarrollo Sustentable (Universidad Nacional de Luján, Consejo Nacional de Investigaciones Científicas y Técnicas), Luján, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Environmental toxicology and chemistry [Environ Toxicol Chem] 2022 May; Vol. 41 (5), pp. 1246-1259. Date of Electronic Publication: 2022 Mar 11.
DOI: 10.1002/etc.5299
Abstrakt: Neotropical fish Cnesterodon decemmaculatus were exposed to different sublethal concentrations (0.5, 1.0 and 5.0 mg As/L) of sodium arsenite (As III) to determine the median lethal concentration (LC50; 96 h) and to evaluate the response of a set of biomarkers (genotoxic, behavioral, biochemical, and metabolic). At the end of the exposure (96 h), fish were video-recorded for behavior assessment. We used the micronucleus and nuclear abnormality tests and the comet assay in peripheral blood as genotoxicity biomarkers. In regard to biochemical and metabolic biomarkers, we dissected the brain for acetylcholinesterase (AChE) activity; the liver for glutathione-S-transferase (GST) and catalase (CAT) activity and glutathione content (GSH); the gills for GSH content; and muscle for AChE, energy metabolism of lipids, carbohydrates, and proteins, and the electron transport system activity of the mitochondrial chain. We calculated an index using metabolic biomarkers, to determine the cellular energy allocation. The LC50 value was 7.32 mg As/L. The As affected some swimming parameters in females. No significant differences in micronucleus were found compared with the control, whereas nuclear aberrations increased significantly at 1.0 and 5.0 mg As/L. The genomic damage index and the percentage of cells with DNA damage (measured by the comet assay) showed a significant increase in the As-treated groups, and this technique was the most sensitive for detecting genotoxic damage. The As affected the antioxidant system (mainly GSH, CAT, and GST) and reduced the lipid content. A preliminary baseline was generated for the response of C. decemmaculatus exposed to sublethal concentrations of As, when it alters swimming behavior and the antioxidant system, has genotoxic effects, and reduces lipid content. Environ Toxicol Chem 2022;41:1246-1259. © 2022 SETAC.
(© 2022 SETAC.)
Databáze: MEDLINE
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