Macrophage calcium reporter mice reveal immune cell communication in vitro and in vivo .

Autor:	Taghdiri N; Department of Bioengineering, Jacobs School of Engineering, University of California, San Diego, 9500 Gilman Dr. MC 0412, La Jolla, CA 92093, USA., Calcagno DM; Department of Bioengineering, Jacobs School of Engineering, University of California, San Diego, 9500 Gilman Dr. MC 0412, La Jolla, CA 92093, USA., Fu Z; Division of Cardiology and Cardiovascular Institute, Department of Medicine, University of California, San Diego, La Jolla, CA, USA., Huang K; Division of Cardiology and Cardiovascular Institute, Department of Medicine, University of California, San Diego, La Jolla, CA, USA., Kohler RH; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge Street, Boston, MA, USA., Weissleder R; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge Street, Boston, MA, USA., Coleman TP; Department of Bioengineering, Jacobs School of Engineering, University of California, San Diego, 9500 Gilman Dr. MC 0412, La Jolla, CA 92093, USA., King KR; Department of Bioengineering, Jacobs School of Engineering, University of California, San Diego, 9500 Gilman Dr. MC 0412, La Jolla, CA 92093, USA.; Division of Cardiology and Cardiovascular Institute, Department of Medicine, University of California, San Diego, La Jolla, CA, USA.; Lead contact.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2021 Dec 20; Vol. 1 (8). Date of Electronic Publication: 2021 Dec 13.
DOI:	10.1016/j.crmeth.2021.100132
Abstrakt:	Cell communication underlies emergent functions in diverse cell types and tissues. Recent evidence suggests that macrophages are organized in communicating networks, but new tools are needed to quantitatively characterize the resulting cellular conversations. Here, we infer cell communication from spatiotemporal correlations of intracellular calcium dynamics that are non-destructively imaged across cell populations expressing genetically encoded calcium indicators. We describe a hematopoietic calcium reporter mouse (Csf1r Cre GCaMP5 fl ) and a computational analysis pipeline for inferring communication between reporter cells based on "excess synchrony." We observed signals suggestive of cell communication in macrophages treated with immune-stimulatory DNA in vitro and tumor-associated immune cells imaged in a dorsal window chamber model in vivo . Together, the methods described here expand the toolkit for discovery of cell communication events in macrophages and other immune cells. Competing Interests: DECLARATION OF INTERESTS The authors declare no competing interests.
Databáze:	MEDLINE
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