Multiple spillovers from humans and onward transmission of SARS-CoV-2 in white-tailed deer.

Autor:	Kuchipudi SV; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802; skuchipudi@psu.edu vkapur@psu.edu.; Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802., Surendran-Nair M; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802.; Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802., Ruden RM; Wildlife Bureau, Iowa Department of Natural Resources, Des Moines, IA 50319.; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011., Yon M; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802., Nissly RH; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802.; Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802., Vandegrift KJ; The Center for Infectious Disease Dynamics, Department of Biology and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802., Nelli RK; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011., Li L; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802., Jayarao BM; Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802., Maranas CD; Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802., Levine N; Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802.; Department of Animal Science, The Pennsylvania State University, University Park, PA 16802., Willgert K; Disease Dynamics Unit, Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom., Conlan AJK; Disease Dynamics Unit, Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom., Olsen RJ; Laboratory of Molecular and Translational Human Infectious Disease Research, Center for Infectious Diseases, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston Methodist Hospital, Houston, TX 77030.; Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10021.; Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021., Davis JJ; University of Chicago Consortium for Advanced Science and Engineering, University of Chicago and Division of Data Science and Learning, Argonne National Laboratory, Lemont, IL 60439., Musser JM; Laboratory of Molecular and Translational Human Infectious Disease Research, Center for Infectious Diseases, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston Methodist Hospital, Houston, TX 77030.; Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10021.; Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021., Hudson PJ; The Center for Infectious Disease Dynamics, Department of Biology and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802., Kapur V; Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802; skuchipudi@psu.edu vkapur@psu.edu.; Department of Animal Science, The Pennsylvania State University, University Park, PA 16802.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Feb 08; Vol. 119 (6).
DOI:	10.1073/pnas.2121644119
Abstrakt:	Many animal species are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and could act as reservoirs; however, transmission in free-living animals has not been documented. White-tailed deer, the predominant cervid in North America, are susceptible to SARS-CoV-2 infection, and experimentally infected fawns can transmit the virus. To test the hypothesis that SARS-CoV-2 is circulating in deer, 283 retropharyngeal lymph node (RPLN) samples collected from 151 free-living and 132 captive deer in Iowa from April 2020 through January of 2021 were assayed for the presence of SARS-CoV-2 RNA. Ninety-four of the 283 (33.2%) deer samples were positive for SARS-CoV-2 RNA as assessed by RT-PCR. Notably, following the November 2020 peak of human cases in Iowa, and coinciding with the onset of winter and the peak deer hunting season, SARS-CoV-2 RNA was detected in 80 of 97 (82.5%) RPLN samples collected over a 7-wk period. Whole genome sequencing of all 94 positive RPLN samples identified 12 SARS-CoV-2 lineages, with B.1.2 ( n = 51; 54.5%) and B.1.311 ( n = 19; 20%) accounting for ∼75% of all samples. The geographic distribution and nesting of clusters of deer and human lineages strongly suggest multiple human-to-deer transmission events followed by subsequent deer-to-deer spread. These discoveries have important implications for the long-term persistence of the SARS-CoV-2 pandemic. Our findings highlight an urgent need for a robust and proactive "One Health" approach to obtain enhanced understanding of the ecology, molecular evolution, and dissemination of SARS-CoV-2. Competing Interests: The authors declare no competing interest. (Copyright © 2022 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu