Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility.

Autor: Costa LVD; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: luciana.costa@bio.fiocruz.br., Miranda RVDSL; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: rebeca.lage@bio.fiocruz.br., Fonseca ELD; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: e.lourofonseca@gmail.com., Gonçalves NP; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: natalia.goncalves@bio.fiocruz.br., Reis CMFD; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: cristhiane.moura@bio.fiocruz.br., Frazão AM; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: adriana.marques@bio.fiocruz.br., Cruz FV; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: fernandaventurac@gmail.com., Brandão MLL; Fundação Oswaldo Cruz, Instituto de Tecnologia em Imunobiológicos (Bio-manguinhos), Microbiological Control Laboratory, Brazil. Electronic address: marcelo.brandao@bio.fiocruz.br., Ramos JN; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz (IOC), Interdisciplinary Medical Research Laboratory, Brazil. Electronic address: jnr.uerj@gmail.com., Vieira VV; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz (IOC), Interdisciplinary Medical Research Laboratory, Brazil. Electronic address: veronicavianavieira@hotmail.com.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2022 Mar; Vol. 194, pp. 106419. Date of Electronic Publication: 2022 Jan 21.
DOI: 10.1016/j.mimet.2022.106419
Abstrakt: VITEK®2, MALDI-TOF MS and 16S rRNA sequencing were evaluated for the identification of aerobic endospore-forming bacteria (AEB) from a pharmaceutical facility. MALDI-TOF MS demonstrated higher accuracy compared to VITEK®2, although both databases were insufficient to identify AEB species. Sequencing was the best methodology, but unable to identify closely related species.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: