Response of substrate kinetics and biological mechanisms to various pH constrains for cultured Nitrobacter and Nitrospira in nitrifying bioreactor.

Autor: Sun H; School of Environmental and Material Engineering, Yantai University, Yantai, Shandong, 264005, China., Zhang H; School of Environmental and Material Engineering, Yantai University, Yantai, Shandong, 264005, China., Zhang F; School of Environmental and Material Engineering, Yantai University, Yantai, Shandong, 264005, China., Yang H; School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China., Lu J; School of Civil Engineering, Yantai University, Yantai, Shandong, 264005, China., Ge S; Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China., Ding J; School of Environmental and Material Engineering, Yantai University, Yantai, Shandong, 264005, China., Liu Y; School of Civil Engineering, Yantai University, Yantai, Shandong, 264005, China. Electronic address: liuyucan@ytu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of environmental management [J Environ Manage] 2022 Apr 01; Vol. 307, pp. 114499. Date of Electronic Publication: 2022 Jan 20.
DOI: 10.1016/j.jenvman.2022.114499
Abstrakt: Nitrite (NO 2 - ) oxidation is an essential step of biological nitrogen cycling in natural ecosystems, and is performed by chemolithoautotrophic nitrite-oxidizing bacteria (NOB). Although Nitrobacter and Nitrospira are regarded as representative NOB in nitrification systems, little attention has focused on kinetic characterisation of the coexistence of Nitrobacter and Nitrospira at various pH values. Here, we evaluate the substrate kinetics, biological mechanism and microbial community dynamics of an enrichment culture including Nitrobacter (17.5 ± 0.9%) and Nitrospira (7.2 ± 0.6%) in response to various pH constrains. Evaluation of the Monod equation at pH 6.0, 6.5, 7.0, 7.5, 8.0 and 8.5 showed that the enrichment had maximum rate (r max ) and maximum substrate affinity (K S ) for NO 2 - oxidation at pH 7.0, which was also supported by the largest absolute abundance of Nitrobacter nxrA (5.26 × 10 7 copies per g wet sludge) and Nitrospira nxrB (1.975 × 10 9 copies per g wet sludge) genes. Moreover, the predominant species for the Nitrobacter-like nxrA were N. vulgaris and N. winogradskyi, while for the Nitrospira-like nxrB, the predominant species were N. japonica, N. calida and Ca. N. bockiana. Furthermore, the r max was strongly and positively correlated with the abundance of the Nitrobacter nxrA or Nitrospira nxrB genes, or N. winogradsk, whereas K S was positively correlated with the abundance of Nitrobacter nxrA or Nitrospira nxrB genes or Ca. N. bockiana. Overall, this study could improve basis kinetic parameters and biological mechanism of NO 2 - oxidation in WWTPs.
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Databáze: MEDLINE
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