| Autor: |
Parker CW; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA., Teixeira MM; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA.; School of Medicine, University of Brasilia, Brasilia 70910-900, Brazil., Singh NK; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA., Raja HA; Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27412, USA., Cank KB; Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27412, USA., Spigolon G; Biological Imaging Facility, California Institute of Technology, Pasadena, CA 91125, USA., Oberlies NH; Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27412, USA., Barker BM; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011, USA., Stajich JE; Department of Microbiology and Plant Pathology, University of California-Riverside, Riverside, CA 92521, USA., Mason CE; WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY 10065, USA., Venkateswaran K; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA. |
| Abstrakt: |
A fungal strain (FJII-L10-SW-P1) was isolated from the Mars 2020 spacecraft assembly facility and exhibited biofilm formation on spacecraft-qualified Teflon surfaces. The reconstruction of a six-loci gene tree (ITS, LSU, SSU, RPB1 and RPB2 , and TEF1 ) using multi-locus sequence typing (MLST) analyses of the strain FJII-L10-SW-P1 supported a close relationship to other known Parengyodontium album subclade 3 isolates while being phylogenetically distinct from subclade 1 strains. The zig-zag rachides morphology of the conidiogenous cells and spindle-shaped conidia were the distinct morphological characteristics of the P. album subclade 3 strains. The MLST data and morphological analysis supported the conclusion that the P. album subclade 3 strains could be classified as a new species of the genus Parengyodontium and placed in the family Cordycipitaceae. The name Parengyodontium torokii sp. nov. is proposed to accommodate the strain, with FJII-L10-SW-P1 as the holotype. The genome of the FJII-L10-SW-P1 strain was sequenced, annotated, and the secondary metabolite clusters were identified. Genes predicted to be responsible for biofilm formation and adhesion to surfaces were identified. Homology-based assignment of gene ontologies to the predicted proteome of P. torokii revealed the presence of gene clusters responsible for synthesizing several metabolic compounds, including a cytochalasin that was also verified using traditional metabolomic analysis. |
