Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel.
| Autor: | Baracho GV; BD Biosciences, 11077 North Torrey Pines Road, La Jolla, CA 92037, USA., Kara N; BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA., Rigaud S; BD Biosciences, 11077 North Torrey Pines Road, La Jolla, CA 92037, USA., Lo E; BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA., Widmann SJ; BD Biosciences, 11077 North Torrey Pines Road, La Jolla, CA 92037, USA., Tyznik AJ; BD Biosciences, 11077 North Torrey Pines Road, La Jolla, CA 92037, USA. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2021 Dec 29; Vol. 3 (1), pp. 101069. Date of Electronic Publication: 2021 Dec 29 (Print Publication: 2022). |
| DOI: | 10.1016/j.xpro.2021.101069 |
| Abstrakt: | Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining workflow for classification of circulating human T and NK cells with cytolytic potential. We suggest reagents for measuring cytolytic proteins and identification of cell subsets within conventional and unconventional T cells and NK cells. Competing Interests: G.V.B., N.K., S.R., S.J.W., and A.J.T. are employees of BD Biosciences. E.L. is a former employee of BD Biosciences. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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