| Autor: |
Atwal HK; Animal Health Centre, British Columbia Ministry of Agriculture, Food and Fisheries, Abbotsford, British Columbia, Canada.; Faculty of Biology, University of British Columbia, Vancouver, British Columbia, Canada., Zabek E; Animal Health Centre, British Columbia Ministry of Agriculture, Food and Fisheries, Abbotsford, British Columbia, Canada., Bidulka J; Animal Health Centre, British Columbia Ministry of Agriculture, Food and Fisheries, Abbotsford, British Columbia, Canada., DuCharme A; SPCA For Monterey County, Salinas, CA, USA., Pawlik M; Animal Health Centre, British Columbia Ministry of Agriculture, Food and Fisheries, Abbotsford, British Columbia, Canada., Himsworth CG; Animal Health Centre, British Columbia Ministry of Agriculture, Food and Fisheries, Abbotsford, British Columbia, Canada.; School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada. |
| Abstrakt: |
Cryptosporidium parvum is a zoonotic, protozoan parasite that causes potentially life-threatening diarrhea in the host and can be transmitted via the fecal-oral route. C. parvum can infect cattle and may be detected in their feces using a variety of tests. We compared the level of agreement, ease of procedure, and cost among PCR, lateral flow immunoassay, fluorescent antibody, and Kinyoun acid-fast stain direct smear tests. Over the course of 9 mo, 74 calf fecal samples were submitted and tested for C. parvum using all 4 tests. A Fleiss kappa value of 0.813 was obtained, indicating an excellent level of agreement among tests. Overall, the best test based on cost and ease of procedure was the Kinyoun acid-fast stain direct smear. |
