Increased bone resorption by long-term cigarette smoke exposure in animal model.

Autor:	Junqueira JJM; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Lourenço JD; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., da Silva KR; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Jorgetti V; Department of Medicine, Laboratory of Renal Physiopathology (LIM-16), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Vieira RP; Post-graduation Program in Sciences of Human Movement and Rehabilitation, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil.; Post-graduation Program in Bioengineering, Brasil University, São Paulo, SP, Brazil.; Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), São José dos Campos, SP, Brazil., de Araujo AA; Exercise Physiology Laboratory, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil., De Angelis K; Exercise Physiology Laboratory, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil., Correia AT; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Alves LHV; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Tibério IFLC; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Barbosa AP; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil., Lopes FDTQDS; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2021 Dec 10; Vol. 7 (12), pp. e08587. Date of Electronic Publication: 2021 Dec 10 (Print Publication: 2021).
DOI:	10.1016/j.heliyon.2021.e08587
Abstrakt:	Introduction: Clinical and experimental studies have been attesting the deleterious effects of smoking mainly due to the stimulation of osteoclastogenesis and inhibition of osteoblastogenesis. However the physiological mechanisms that can explain these changes are not fully understood. Aims: To evaluate the trabecular bone resorption effect caused by long-term exposure to cigarette smoke and the action of cytokines and reactive oxygen species involved in this process. Methods: Sixty young adult C57BL/6 mice were allocated to two groups: control, 30 animals exposed to filtered air for 1, 3 and 6 months; and smoke, 30 animals exposed to cigarette smoke for 1, 3 and 6 months. Femoral and tibial extraction was performed to evaluate the bone mineral matrix, bone cytokines (Receptor activator of nuclear factor-kappa B ligand - RANKL and Osteoprotegerin - OPG) and oxidative stress markers (Thiobarbituric acid reactive substances - Tbars). Results: Exposure to cigarette smoke (CS) generated changes in bone structural parameters in the 6th month of follow-up, demonstrating an evident bone loss; reduction in OPG/RANKL ratio from the 3rd month on and increase in Tbars in the first month, both closely related to the increase in osteoclastogenic activity and bone resorption. Conclusion: These findings reinforce the importance of CS-induced oxidative stress in bone compromising the bone cellular activities with a consequent impairment in bone turn over and changes in bone structure. Competing Interests: The authors declare no conflict of interest. (© 2021 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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